Academic Journal
Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans
| العنوان: | Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans |
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| المؤلفون: | Larissa L.S. Scholte, David J. Leggat, Kristen W. Cohen, Lara Hoeweler, Guacyara C. Erwin, Farhard Rahaman, Angela Lombardo, Vincent Philiponis, Dagna S. Laufer, Heather Siefers, Alexis M. Ruppel, Joshua Brand, Janine Maenza, Rhi Bronson, Madhu Prabhakaran, Jalen Jean-Baptiste, Orpheus Kolokythas, Aimee A. Desrosiers, Caroline K. Thoreson, Antje Heit, Nadia J. Khati, Elissa Malkin, M. Juliana McElrath, Adrian B. McDermott, William R. Schief, David Diemert, Jeffrey M. Bethony |
| المصدر: | STAR Protocols, Vol 4, Iss 4, Pp 102576- (2023) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Cell Isolation, Clinical Protocol, Immunology, Science (General), Q1-390 |
| الوصف: | Summary: The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy.For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2666-1667 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2666166723005439; https://doaj.org/toc/2666-1667; https://doaj.org/article/35d5c65745b04af5bc934414faf8dd90 |
| DOI: | 10.1016/j.xpro.2023.102576 |
| الاتاحة: | https://doi.org/10.1016/j.xpro.2023.102576 https://doaj.org/article/35d5c65745b04af5bc934414faf8dd90 |
| رقم الانضمام: | edsbas.6AB226A3 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 26661667 |
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| DOI: | 10.1016/j.xpro.2023.102576 |