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Non-amyloid aggregate formation underlies the increased Sup35 aggregation observed in btn2 hsp42 mutants.
| العنوان: | Non-amyloid aggregate formation underlies the increased Sup35 aggregation observed in btn2 hsp42 mutants. |
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| المؤلفون: | Zorana Carter, Declan Creamer, Aikaterini Kouvidi, Chris M. Grant |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Neuroscience, Evolutionary Biology, Cancer, Inorganic Chemistry, Infectious Diseases, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, quality control mechanisms, play key roles, div >< p, >< sup ><, sequester oxidized proteins, prion formation occurs, multiple intracellular sites, heritable prion form, insoluble protein deposit, sequestrase chaperones protect, oxidative stress conditions, exact intracellular functions, induced protein aggregation, amyloid sup35 aggregates, btn2 hsp42 <, oxidative stress, induced formation, amyloid form |
| الوصف: | A. [ PSI + ] prion formation was quantified in the wild-type, abp1 , btn2 hsp42 and btn2 hsp42 abp1 mutant strains during non-stress conditions and following exposure to 0.8 mM hydrogen peroxide for one hour. Data shown are the means of at least three independent biological repeat experiments expressed as the number of colonies per 10 5 viable cells. Error bars denote standard deviation. Significance is shown using a one-way ANOVA test; * p < 0.05 *** p < 0.001. B. SDS-resistant Sup35 and Rnq1 aggregates were detected in the wild-type and btn2 hsp42 mutant strains using SDD-AGE. Strains were grown to exponential phase and left untreated (-) or treated with with 0.8 mM hydrogen peroxide (+) for one hour. [ psi - ] and rnq1 deletion strains are shown for comparison with Rnq1 and [ PSI + ] and [ psi - ] strains are shown for comparison with Sup35. Aggregate and monomer (M) forms are indicated. C. Representative epifluorescent microscopic images are shown from strains expressing Sup35-GFP or ΔN-Sup35-GFP. Strains were grown to exponential phase and left untreated (non-stress) or treated with with 0.8 mM hydrogen peroxide for one hour. Charts show the percentage of cells contain 0, 1–3, or >3 Sup35 puncta per cell scored in 300 cells for each strain. Significance: ** p < 0.01, *** p < 0.001 (Mann–Whiney U-test). |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Non-amyloid_aggregate_formation_underlies_the_increased_Sup35_aggregation_observed_in_i_btn2_i_hsp42_mutants_/25320270 |
| DOI: | 10.1371/journal.pgen.1011194.g006 |
| الاتاحة: | https://doi.org/10.1371/journal.pgen.1011194.g006 https://figshare.com/articles/figure/Non-amyloid_aggregate_formation_underlies_the_increased_Sup35_aggregation_observed_in_i_btn2_i_hsp42_mutants_/25320270 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.6A6CED27 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pgen.1011194.g006 |
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