Academic Journal
Nanofat Improves Vascularization and Tissue Integration of Dermal Substitutes without Affecting Their Biocompatibility.
| العنوان: | Nanofat Improves Vascularization and Tissue Integration of Dermal Substitutes without Affecting Their Biocompatibility. |
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| المؤلفون: | Bonomi, F., Limido, E., Weinzierl, A., Ampofo, E., Harder, Y., Menger, M.D., Laschke, M.W. |
| المصدر: | Journal of functional biomaterials, vol. 15, no. 10 |
| سنة النشر: | 2024 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Integra®, angiogenesis, dermal substitutes, inflammation, nanofat, skin regeneration, vascularization |
| الوصف: | Dermal substitutes require sufficient tissue integration and vascularization to be successfully covered with split-thickness skin grafts. To rapidly achieve this, we provide the proof of principle for a novel vascularization strategy with high translational potential. Nanofat was generated from subcutaneous adipose tissue of green fluorescence protein (GFP) + C57BL/6J donor mice and seeded onto small samples (4 mm in diameter) of the clinically approved dermal substitute Integra ® . These samples and non-seeded controls were then implanted into full-thickness skin defects in the dorsal skinfold chamber of C57BL/6J wild-type mice and analyzed by intravital fluorescence microscopy, histology and immunohistochemistry over a 14-day period. Nanofat-seeded dermal substitutes exhibited an accelerated vascularization, as indicated by a significantly higher functional microvessel density on days 10 and 14 when compared to controls. This was primarily caused by the reassembly of GFP + microvascular fragments inside the nanofat into microvascular networks. The improved vascularization promoted integration of the implants into the surrounding host tissue, which finally exhibited an increased formation of a collagen-rich granulation tissue. There were no marked differences in the inflammatory host tissue reaction to nanofat-seeded and control implants. These findings demonstrate that nanofat significantly improves the in vivo performance of dermal substitutes without affecting their biocompatibility. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/39452592; info:eu-repo/semantics/altIdentifier/pissn/2079-4983; https://serval.unil.ch/notice/serval:BIB_AE9868E84D8C; https://serval.unil.ch/resource/serval:BIB_AE9868E84D8C.P001/REF.pdf |
| DOI: | 10.3390/jfb15100294 |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_AE9868E84D8C https://doi.org/10.3390/jfb15100294 https://serval.unil.ch/resource/serval:BIB_AE9868E84D8C.P001/REF.pdf |
| Rights: | info:eu-repo/semantics/openAccess ; CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.6A2D6A54 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/jfb15100294 |
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