Academic Journal
A Modified Basophil Activation Test for the Clinical Management of Immediate Hypersensitivity Reactions to Paclitaxel: A Proof-of-Concept Study
| العنوان: | A Modified Basophil Activation Test for the Clinical Management of Immediate Hypersensitivity Reactions to Paclitaxel: A Proof-of-Concept Study |
|---|---|
| المؤلفون: | Marilena La Sorda, Marco Fossati, Rosalia Graffeo, Manuela Ferraironi, Maria Cristina De Rosa, Alexia Buzzonetti, Benedetta Righino, Nicole Zampetti, Andrea Fattorossi, Eleonora Nucera, Arianna Aruanno, Gabriella Ferrandina, Adriana Ionelia Apostol, Alessandro Buonomo, Giovanni Scambia, Maurizio Sanguinetti, Alessandra Battaglia |
| المصدر: | Cancers, Vol 15, Iss 24, p 5818 (2023) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | basophils, BAT, flow cytometry, hypersensitivity, MRGPRX2, platinum, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Immediate hypersensitivity reactions (iHSRs) to taxanes are observed in 6% and 4% of gynecologic and breast cancer patients, respectively. Drug desensitization is the only option, as no comparable alternative therapy is available. Surfactants in the taxane formulation have been implicated in the immunopathogenesis of iHSRs, although sporadic skin test (ST) positivity and iHSRs to nab-paclitaxel have suggested the involvement of the taxane moiety and/or IgE-mediated pathomechanisms. In vitro diagnostic tests might offer insights into mechanisms underlying iHSRs to taxanes. The aim of the present study was to address this unmet need by developing a novel basophil activation test (BAT). The study included patients ( n = 31) undergoing paclitaxel/carboplatin therapy. Seventeen patients presented with iHSRs to paclitaxel (iHSR-Tax pos ), and eleven were tolerant (iHSR-Tax neg ). Fourteen patients presented with iHSRs to carboplatin (iHSR-Pl pos ), and fourteen were tolerant (iHSR-Pl neg ). The BAT median stimulation index (SI) values were 1.563 (range, 0.02–4.11; n = 11) and −0.28 (range −4.88–0.07, n = 11) in iHSR-Tax pos and iHSR-Tax neg , respectively. The BAT median SI values were 4.45 (range, 0.1–26.7; n = 14) and 0 (range, −0.51–1.65; n = 12) in iHSR-Pl pos and iHSR-Pl neg , respectively. SI levels were not associated with iHSR severity grading. Comparing BAT results in iHSR-Tax pos and iHSR-Tax neg showed the area under the receiver operator characteristic (ROC) curve to be 0.9752 ( p = 0.0002). The cutoff calculated by the maximized likelihood ratio identified 90.91% of iHSR-Tax pos patients and 90.91% of iHSR-Tax neg patients. Comparing BAT results for iHSR-Pl pos and iHSR-Pl neg showed the area under the ROC curve to be 0.9286 ( p = 0.0002). The cutoff calculated by the maximized likelihood ratio identified 78.57% of iHSR-Pl pos patients and 91.67% of iHSR-Pl neg patients. Most iHSR-Tax pos patients for which ST was available (10/11) scored ST-negative and BAT-positive, whereas most iHSR-Pl pos patients for ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2072-6694 |
| Relation: | https://www.mdpi.com/2072-6694/15/24/5818; https://doaj.org/toc/2072-6694; https://doaj.org/article/b17b91092bce4f359b5582f966268720 |
| DOI: | 10.3390/cancers15245818 |
| الاتاحة: | https://doi.org/10.3390/cancers15245818 https://doaj.org/article/b17b91092bce4f359b5582f966268720 |
| رقم الانضمام: | edsbas.6A17802C |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20726694 |
|---|---|
| DOI: | 10.3390/cancers15245818 |