Academic Journal
Reprogramming Microbial CO2-Metabolizing Chassis With CRISPR-Cas Systems
| العنوان: | Reprogramming Microbial CO2-Metabolizing Chassis With CRISPR-Cas Systems |
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| المؤلفون: | Yu, Hai-Yan, Wang, Shu-Guang, Xia, Peng-Fei |
| المساهمون: | National Natural Science Foundation of China, Shandong University, Natural Science Foundation of Shandong Province |
| المصدر: | Frontiers in Bioengineering and Biotechnology ; volume 10 ; ISSN 2296-4185 |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2022 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | Global warming is approaching an alarming level due to the anthropogenic emission of carbon dioxide (CO 2 ). To overcome the challenge, the reliance on fossil fuels needs to be alleviated, and a significant amount of CO 2 needs to be sequestrated from the atmosphere. In this endeavor, carbon-neutral and carbon-negative biotechnologies are promising ways. Especially, carbon-negative bioprocesses, based on the microbial CO 2 -metabolizing chassis, possess unique advantages in fixing CO 2 directly for the production of fuels and value-added chemicals. In order to fully uncover the potential of CO 2 -metabolizing chassis, synthetic biology tools, such as CRISPR-Cas systems, have been developed and applied to engineer these microorganisms, revolutionizing carbon-negative biotechnology. Herein, we review the recent advances in the adaption of CRISPR-Cas systems, including CRISPR-Cas based genome editing and CRISPR interference/activation, in cyanobacteria, acetogens, and methanogens. We also envision future innovations via the implementation of rising CRISPR-Cas systems, such as base editing, prime editing, and transposon-mediated genome editing. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fbioe.2022.897204 |
| DOI: | 10.3389/fbioe.2022.897204/full |
| الاتاحة: | http://dx.doi.org/10.3389/fbioe.2022.897204 https://www.frontiersin.org/articles/10.3389/fbioe.2022.897204/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.69FB263B |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fbioe.2022.897204 |
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