Academic Journal
A new electrospray method for targeted gene delivery.
| العنوان: | A new electrospray method for targeted gene delivery. |
|---|---|
| المؤلفون: | Boehringer, Stephan, Ruzgys, Paulius, Tamò, Luca Giuseppe Athos, Šatkauskas, Saulius, Geiser, Thomas, Gazdhar, Amiq, Hradetzky, David |
| المصدر: | Boehringer, Stephan; Ruzgys, Paulius; Tamò, Luca Giuseppe Athos; Šatkauskas, Saulius; Geiser, Thomas; Gazdhar, Amiq; Hradetzky, David (2018). A new electrospray method for targeted gene delivery. Scientific Reports, 8(1), p. 4031. Nature Publishing Group 10.1038/s41598-018-22280-2 |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2018 |
| المجموعة: | BORIS (Bern Open Repository and Information System, University of Bern) |
| مصطلحات موضوعية: | 610 Medicine & health |
| الوصف: | A challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanism. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://boris.unibe.ch/112618/ |
| الاتاحة: | https://boris.unibe.ch/112618/1/Boehringer%20SciRep%202018.pdf https://boris.unibe.ch/112618/ |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.69F2B646 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |