Academic Journal
The Legionella pneumophila orphan sensor kinase LqsT regulates competence and pathogen-host interactions as a component of the LAI-1 circuit.
| العنوان: | The Legionella pneumophila orphan sensor kinase LqsT regulates competence and pathogen-host interactions as a component of the LAI-1 circuit. |
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| المؤلفون: | Kessler, Aline, Schell, Ursula, Sahr, Tobias, Tiaden, André, Harrison, Christopher, Buchrieser, Carmen, Hilbi, Hubert |
| المساهمون: | Max Von Pettenkofer Institute (MVP), Ludwig Maximilian University Munich = Ludwig Maximilians Universität München (LMU), Biologie des bactéries intracellulaires, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Center for Applied Biotechnology and Molecular Medicine (CABMM), Universität Zürich Zürich = University of Zurich (UZH), Work in the group of H.H. was supported by the Max von Pettenkofer Institute, Ludwig-Maximilians University Munich, the German Research Foundation (DFG, HI 1511/2-1, SPP 1617) and the Swiss National Science Foundation (SNF, 31003A_125369 Sinergia, CRSI33_130016)., C.B. received funding from the Institut Pasteur, the LabEx program IBEID, the Centre National de la Recherche Scientifique (CNRS), the Institut Carnot-Pasteur MI, the Fondation pour la Recherche Médicale (FRM), the French region Ile-de-France (DIM Malinf) and from the ANR-10-PATH-004 project, in the frame of ERA-Net PathoGenoMics., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-PATH-0004,MobileGenomics(2010) |
| المصدر: | ISSN: 1462-2912. |
| بيانات النشر: | HAL CCSD Society for Applied Microbiology and Wiley-Blackwell |
| سنة النشر: | 2013 |
| المجموعة: | Institut Pasteur: HAL |
| مصطلحات موضوعية: | MESH: Acanthamoeba castellanii/microbiology, MESH: Bacterial Proteins/genetics, MESH: Gene Deletion, MESH: Gene Expression Regulation, Bacterial, MESH: Host-Pathogen Interactions/physiology, MESH: Legionella pneumophila/drug effects, MESH: Legionella pneumophila/genetics, MESH: Legionella pneumophila/growth & development, MESH: Legionella pneumophila/physiology, MESH: Phosphotransferases/genetics, MESH: Phosphotransferases/metabolism, MESH: Quorum Sensing/genetics, MESH: Salts/pharmacology, MESH: Transcription Factors/metabolism, MESH: Bacterial Proteins/metabolism, MESH: DNA Transformation Competence/genetics, [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| الوصف: | International audience ; Legionella pneumophila is an amoeba-resistant opportunistic pathogen that performs cell-cell communication through the signalling molecule 3-hydroxypentadecane-4-one (LAI-1, Legionella autoinducer-1). The lqs (Legionella quorum sensing) gene cluster encodes the LAI-1 autoinducer synthase LqsA, the cognate sensor kinase LqsS and the response regulator LqsR. Here we show that the Lqs system includes an 'orphan' homologue of LqsS termed LqsT. Compared with wild-type L. pneumophila, strains lacking lqsT or both lqsS and lqsT show increased salt resistance, greatly enhanced natural competence for DNA acquisition and impaired uptake by phagocytes. Sensitive novel single round growth assays and competition experiments using Acanthamoeba castellanii revealed that ΔlqsT and ΔlqsS-ΔlqsT, as well as ΔlqsA and other lqs mutant strains are impaired for intracellular growth and cannot compete against wild-type bacteria upon co-infection. In contrast to the ΔlqsS strain, ΔlqsT does not produce extracellular filaments. The phenotypes of the ΔlqsS-ΔlqsT strain are partially complemented by either lqsT or lqsS, but are not reversed by overexpression of lqsA, suggesting that LqsT and LqsS are the sole LAI-1-responsive sensor kinases in L. pneumophila. In agreement with the different phenotypes of the ΔlqsT and ΔlqsS strains, lqsT and lqsS are differentially expressed in the post-exponential growth phase, and transcriptome studies indicated that 90% of the genes, which are downregulated in absence of lqsT, are upregulated in absence of lqsS. Reciprocally regulated genes encode components of a 133 kb genomic 'fitness island' or translocated effector proteins implicated in virulence. Together, these results reveal a unique organization of the L. pneumophila Lqs system comprising two partially antagonistic LAI-1-responsive sensor kinases, LqsT and LqsS, which regulate distinct pools of genes implicated in pathogen-host cell interactions, competence, expression of a genomic island or production of extracellular ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/23033905; pasteur-01336729; https://pasteur.hal.science/pasteur-01336729; PUBMED: 23033905 |
| DOI: | 10.1111/j.1462-2920.2012.02889.x |
| الاتاحة: | https://pasteur.hal.science/pasteur-01336729 https://doi.org/10.1111/j.1462-2920.2012.02889.x |
| رقم الانضمام: | edsbas.6990ECDC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1462-2920.2012.02889.x |
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