Academic Journal
Molecular and serological detection of arthropod-borne pathogens in carnivorous birds from Brazil
| العنوان: | Molecular and serological detection of arthropod-borne pathogens in carnivorous birds from Brazil |
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| المؤلفون: | Sacchi, Ana Beatriz Vieira, André, Marcos Rogério, Calchi, Ana Cláudia, de Santi, Mariele, Guimarães, Andresa, Pires, Jeferson Rocha, Baldani, Cristiane Divan, Werther, Karin, Machado, Rosangela Zacarias |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| سنة النشر: | 2021 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | Anaplasmataceae, Avian, Bartonellaceae, Haemosporida |
| الوصف: | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) ; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ; Processo FAPESP: 2012/11870-3 ; CNPq: 302420/2017-7 ; CNPq: 306326/2014-0 ; Rickettsiales, Haemosporida and Rhizobiales agents can cause diseases that affect various animal species, including humans. Due to predation behaviour, carnivorous birds may play an important role in spreading these etiological agentes across geographically distant areas, specially if they are migratory. The aim of this study was to investigate the occurrence and to access the phylogenetic relations among Anaplasmataceae (Ehrlichia, Anaplasma, Neorickettsia), Bartonellaceae (Bartonella spp.), and Haemosporida (Plasmodium, Haemoproteus and Leucocytozoon) agents in blood samples from 121 carnivorous birds sampled in the states of São Paulo and Rio de Janeiro, Brazil. Inclusions resembling hemoparasites were not observed in Giemsa-stained preparations. While three animals were seropositive for E. chaffeensis (3.41% [3/88]; 95% CI:1.17–9.55%), five showed antibodies to A. phagocytophilum (5.68% [5/88]; 95% CI: 2.45–12.62%). Despite the detection of rrs gene fragments closely related to E. chaffeensis (4.13% [5/121]; 95% CI: 1.78–9.31%), no positivity was observed in the qPCR based on the genes vlpt for the organism. Similarly, 12 (9.91% [12/121]; 95% CI: 5.76–16.74%) samples were positive in the qPCR for Anaplasma spp. based on groEL gene, but negative in the qPCR for A. phagocytophilum based on msp-2 gene. Three samples were positive in the nPCR for E. canis based on rrs gene. Three samples were positive for Haemoproteus spp. and one for Plasmodium spp. in the nPCR based on cytB gene. Four birds (3.3% [4/121]; 95% CI: 1.29–8.19%) presented co-positivity by Ehrlichia sp. and Anaplasma sp. in molecular assays. One (0.82% [1/121]; 95% CI:0.15–4.53%) bird showed to be seropositive for E. chaffeensis and and positive in PCR for Haemoproteus sp. All birds were negative in the qPCR assay for Bartonella spp. (nuoG). ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2405-9390 |
| Relation: | Veterinary Parasitology: Regional Studies and Reports; http://dx.doi.org/10.1016/j.vprsr.2021.100539; Veterinary Parasitology: Regional Studies and Reports, v. 23.; http://hdl.handle.net/11449/207262; 2-s2.0-85100714148 |
| DOI: | 10.1016/j.vprsr.2021.100539 |
| الاتاحة: | http://hdl.handle.net/11449/207262 https://doi.org/10.1016/j.vprsr.2021.100539 |
| رقم الانضمام: | edsbas.69804886 |
| قاعدة البيانات: | BASE |
| تدمد: | 24059390 |
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| DOI: | 10.1016/j.vprsr.2021.100539 |