Academic Journal
Maternal ageing impairs mitochondrial DNA kinetics during early embryogenesis in mice
| العنوان: | Maternal ageing impairs mitochondrial DNA kinetics during early embryogenesis in mice |
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| المؤلفون: | May-Panloup, P, Brochard, V, Hamel, J F, Desquiret-Dumas, V, Chupin, S, Reynier, P, Duranthon, V |
| المساهمون: | INRA, CNRS, Inserm, University of Angers |
| المصدر: | Human Reproduction ; volume 34, issue 7, page 1313-1324 ; ISSN 0268-1161 1460-2350 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2019 |
| الوصف: | STUDY QUESTION Does ageing affect the kinetics of the mitochondrial pool during oogenesis and early embryogenesis? SUMMARY ANSWER While we found no age-related change during oogenesis, the kinetics of mitochondrial DNA content and the expression of the factors involved in mitochondrial biogenesis appeared to be significantly altered during embryogenesis. WHAT IS KNOWN ALREADY Oocyte mitochondria are necessary for embryonic development. The morphological and functional alterations of mitochondria, as well as the qualitative and quantitative mtDNA anomalies, observed during ovarian ageing may be responsible for the alteration of oocyte competence and embryonic development. STUDY DESIGN, SIZE, DURATION The study, conducted from November 2016 to November 2017, used 40 mice aged 5–8 weeks and 45 mice aged 9–11 months (C57Bl6/CBA F(1)). A total of 488 immature oocytes, with a diameter ranging from 20 μm to more than 80 μm, were collected from ovaries, and 1088 mature oocytes or embryos at different developmental stages (two PN, one-cell, i.e. syngamy, two-cell, four-cell, eight-cell, morula and blastocyst) were obtained after ovarian stimulation and, for embryos, mating. PARTICIPANTS/MATERIALS, SETTING, METHODS Mitochondrial DNA was quantified by quantitative PCR. We used quantitative reverse transcriptase PCR (RT-PCR) (microfluidic method) to study the relative expression of three genes involved in the key steps of embryogenesis, i.e. embryonic genome activation (HSPA1) and differentiation (CDX2 and NANOG), two mtDNA genes (CYB and ND2) and five genes essential for mitochondrial biogenesis (PPARGC1A, NRF1, POLG, TFAM and PRKAA). The statistical analysis was based on mixed linear regression models applying a logistic link function (STATA v13.1 software), with values of P < 0.05 being considered significant. MAIN RESULTS AND THE ROLE OF CHANCE During oogenesis, there was a significant increase in oocyte mtDNA content (P < 0.0001) without any difference between the two groups of mice (P = 0.73). During ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/humrep/dez054 |
| DOI: | 10.1093/humrep/dez054/28877160/dez054.pdf |
| الاتاحة: | https://doi.org/10.1093/humrep/dez054 http://academic.oup.com/humrep/advance-article-pdf/doi/10.1093/humrep/dez054/28877160/dez054.pdf http://academic.oup.com/humrep/article-pdf/34/7/1313/28911474/dez054.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.69567813 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/humrep/dez054 |
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