Academic Journal
Hydroalcoholic extract of Tagetes minuta L. inhibits inflammatory bowel disease through the activity of pheophytins on the NF-κB signalling pathway
| العنوان: | Hydroalcoholic extract of Tagetes minuta L. inhibits inflammatory bowel disease through the activity of pheophytins on the NF-κB signalling pathway |
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| المؤلفون: | Apaza Ticona, Luis, Lacheva, Ginka Ilieva, Serban, Andreea Madalina, Rumbero Sánchez, Ángel |
| المساهمون: | UAM. Departamento de Química Orgánica |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | Universidad Autónoma de Madrid (UAM): Biblos-e Archivo |
| مصطلحات موضوعية: | Anti-inflammatory, Pheophytins, Tagetes minuta, NF-κB, TNF-α, Química |
| الوصف: | Ethno-pharmacological relevance: Species of the genus Tagetes are well known for their anti-inflammatory properties. Tagetes minuta “Huacatay” is an endemic species of South America that has been used in traditional medicine since ancient times as a remedy for stomach and intestinal discomfort. Aim of the study: The aim of this study is to investigate the anti-inflammatory activity of the aqueous and hydroalcoholic extracts of the Huacatay, identifying the compounds responsible for this activity. Materials and methods: Anti-inflammatory activity of the compounds, fractions and extracts was evaluated in Hs 746T (stomach), HIEC-6 (intestine) and THP-1 (monocytes peripheral blood) cells by measuring their inhibitory capacity against the NF-κB production. Results: Aqueous and hydroalcoholic extracts of Tagetes minuta displayed anti-inflammatory activity in vitro, the hydroalcoholic extract being the most active (IC50 between 59.72 and 66.42 μg/mL) in all cell lines. Bio-guided hydroalcoholic extract fractionation led to the isolation and characterisation of two pheophytins, pheophytin a (1) and 132-hydroxy pheophytin a (2). Both compounds inhibited the production of NF-κB with IC50 values in the low micromolar range, with an IC50 between 12.32 and 16.01 μM for compound 1 and 7.91–9.87 μM for compound 2. Conclusions: The two pheophytins isolated in this study inhibit the production of NF-κB, thus showing that the traditional anti-inflammatory use of Tagetes minuta can be proved through pharmacological assays. This contributes to understanding the anti-inflammatory activity of the Huacatay extracts and their use in the treatment of stomach and intestinal discomfort ; This work was supported by the National Herbarium of Bolivia and the Fundación de la Universidad Autónoma de Madrid (FUAM) |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Journal of Ethnopharmacology; https://doi.org/10.1016/j.jep.2020.113603; Journal of Ethnopharmacology 268 (2021): 113603; 0378-8741 (print); http://hdl.handle.net/10486/699580; 113603-1; 113603-27; 2021 |
| DOI: | 10.1016/j.jep.2020.113603 |
| الاتاحة: | http://hdl.handle.net/10486/699580 https://doi.org/10.1016/j.jep.2020.113603 |
| Rights: | © 2020 Elsevier B.V. ; Reconocimiento – NoComercial – SinObraDerivada ; openAccess |
| رقم الانضمام: | edsbas.692A59F4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jep.2020.113603 |
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