التفاصيل البيبلوغرافية
| العنوان: |
A TLR7/8 Agonist-Including DOEPC-Based Cationic Liposome Formulation Mediates Its Adjuvanticity Through the Sustained Recruitment of Highly Activated Monocytes in a Type I IFN-Independent but NF-κB-Dependent Manner |
| المؤلفون: |
Auderset, Floriane, Belnoue, Elodie, Mastelic-Gavillet, Beatris, Lambert, Paul Henri, Siegrist, Claire-Anne |
| المصدر: |
ISSN: 1664-3224 ; Frontiers in immunology, vol. 11 (2020) 580974. |
| سنة النشر: |
2020 |
| المجموعة: |
Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: |
info:eu-repo/classification/ddc/616.07, info:eu-repo/classification/ddc/618, TLR7/8 agonist, Adjuvants for vaccine, Follicular T helper cells, Germinal centers, Liposome, Adjuvants, Immunologic / administration & dosage, Animals, B-Lymphocytes / immunology, Dendritic Cells / immunology, Drug Compounding, Germinal Center / immunology, Heterocyclic Compounds, 3-Ring / administration & dosage, Immunity, Innate, Interferon Type I / immunology, Ligands, Liposomes / administration & dosage, Membrane Glycoproteins / agonists, Mice, Inbred C57BL, Knockout, Monocytes / immunology, NF-kappa B / deficiency, NF-kappa B / genetics, NF-kappa B / immunology, Phosphatidylcholines / administration & dosage |
| الوصف: |
Novel adjuvants, such as Toll-like receptors (TLRs) agonists, are needed for the development of new formulations able to circumvent limitations of current vaccines. Among TLRs, TLR7/8 agonists represent promising candidates, as they are well described to enhance antigen-specific antibody responses and skew immunity toward T helper (T H ) 1 responses. We find here that the incorporation of the synthetic TLR7/8 ligand 3M-052 in a cationic DOEPC-based liposome formulation shifts immunity toward T H 1 responses and elicits strong and long-lasting germinal center and follicular T helper cell responses in adult mice. This reflects the prolonged recruitment of innate cells toward the site of immunization and homing of activated antigen-loaded monocytes and monocyte-derived dendritic cells toward draining lymph nodes. We further show that this adjuvanticity is independent of type I IFN but NF-κB-dependent. Overall, our data identify TLR7/8 agonists incorporated in liposomes as promising and effective adjuvants to enhance T H 1 and germinal center responses. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/33262759; https://archive-ouverte.unige.ch/unige:156474; unige:156474 |
| الاتاحة: |
https://archive-ouverte.unige.ch/unige:156474 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.688E0F37 |
| قاعدة البيانات: |
BASE |