التفاصيل البيبلوغرافية
| العنوان: |
Development of the A/H6N1 influenza vaccine candidate based on A/Leningrad/134/17/57 (H2N2) master donor virus and the genome composition analysis using high resolution melting (HRM) |
| المؤلفون: |
Desheva Yulia A., Smolonogina Tatiana A., Landgraf Galina O., Rudenko Larisa G. |
| المصدر: |
Microbiology Independent Research Journal, 3(1), 61-67, (2016-09-16) |
| بيانات النشر: |
Zenodo |
| سنة النشر: |
2016 |
| المجموعة: |
Zenodo |
| مصطلحات موضوعية: |
influenza virus, live influenza vaccine, HRM-analysis |
| الوصف: |
The avian influenza viruses of H6N1subtype present a potential danger for humans. The cold-adapted ( ca ) reassortant influenza virus А/17/herring gull/Sarma/2006/887 (H6N1) was obtained in chicken embryos by the genetic reassortment based on the cold-adapted A/Leningrad/134/17/57 (H2N2) master strain. The genome composition of the obtained reassortant was analyzed by means of real-time PCR with the high resolution melting (HRM) analysis using the intercalating fluorescent dye EvaGreen. Analysis of the gene segments showed that the reassortant А/17/herring gull/Sarma/2006/887 (H6N1) contains the internal proteins coding genes (PB2, PB1, PA, NP, M, and NS) of the master donor virus and the surface antigens coding genes of the A/herring gull/Sarma/51c/2006 (H6N1) avian influenza virus. The study of the phenotypic properties showed that the virus А/17/herring gull/Sarma/2006/887 (H6N1) is temperature sensitive ( ts ), ca in chicken embryos, and attenuated in mice when administered intranasally. This reassortant can be recommended as a live influenza vaccine candidate for humans. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
oai:zenodo.org:3858067 |
| DOI: |
10.18527/2500-2236-2016-3-1-61-67 |
| الاتاحة: |
https://doi.org/10.18527/2500-2236-2016-3-1-61-67 |
| Rights: |
info:eu-repo/semantics/openAccess ; Creative Commons Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/legalcode |
| رقم الانضمام: |
edsbas.68650D5F |
| قاعدة البيانات: |
BASE |