Academic Journal
Identification of an interferon-stimulated gene, isg15, involved in host immune defense against viral infections in gilthead seabream (Sparus aurata L.)
| العنوان: | Identification of an interferon-stimulated gene, isg15, involved in host immune defense against viral infections in gilthead seabream (Sparus aurata L.) |
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| المؤلفون: | Alvarez-Torres, D., Gómez-Abellán, V. (Victoria), Arizcun-Arizcun, M. (Marta), García-Rosado, E., Béjar, J., Sepulcre, M.P. (Maria Pilar) |
| بيانات النشر: | Centro Oceanográfico de Murcia |
| سنة النشر: | 2017 |
| المجموعة: | Instituto Español de Oceanografía: e-IEO |
| مصطلحات موضوعية: | Teleost, isg15 gene, Antiviral activity, SJNNV, VHSV, IFN I, Phagocytes, fish, immunology, length, identification |
| الوصف: | Interferons (IFNs) play a key role in the innate immunity of vertebrates against viral infections by inducing hundreds of IFN-stimulated genes (ISGs), such as isg15. Isg15 is an ubiquitin-like protein, which can conjugate cellular and viral proteins in a process called ISGylation, although it can also act as a cytokine-like protein. Gilthead seabream (Sparus aurata L.) is an important asymptomatic carrier of viral haemorrhagic septicaemia virus (VHSV) and nodavirus, representing a threat to other co-cultivated susceptible species. In order to better understand virus-host interactions in this fish species, this study addresses the identification and molecular characterization of seabream isg15 (sb-isg15). In addition, the modulation of transcript levels of sb-isg15 was analysed in SAF-1 cells and seabream acidophilic granulocytes (AGs) stimulated in vitro with different pathogenassociated molecular patterns (PAMPs) or inoculated with VHSV and striped jack nervous necrosis virus (SJNNV). The full-length cDNA of sb-isg15 gene, encoding a predicted protein of 155 amino acids, was identified and seen to share the same characteristics as other fish and mammalian isg15 genes. Here we report the clear induction of sb-isg15 transcript levels in SAF-1 cells and AGs stimulated with toll-like receptor (TLR) ligands, such as polyinosinic:polycytidylic acid (poly I:C) or genomic DNA from Vibrio anguillarum (VaDNA), respectively. Furthermore, VHSV and SJNNV inoculation induced a significant degree of sb-isg15 transcription in SAF-1 cells and AGs. However, the relative levels of viral RNA transcription showed that SJNNV replication seems to be more efficient than VHSV in both in vitro systems. Interestingly, sb-isg15 transcript induction elicited by VaDNA was reduced in VHSV- and SJNNV-inoculated AGs, suggesting an interference prompted by the viruses against the type I IFN system. Taken together, these findings support the use of seabream AGs as a valuable experimental system to study virus-host interactions, in which sb-isg15 ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1050-4648 |
| Relation: | https://www.sciencedirect.com/science/article/abs/pii/S1050464817307684?via%3Dihub; http://hdl.handle.net/10508/11782; Fish and Shellfish Immunology, 73. 2017: 220-227 |
| DOI: | 10.1016/j.fsi.2017.12.027 |
| الاتاحة: | http://hdl.handle.net/10508/11782 https://doi.org/10.1016/j.fsi.2017.12.027 |
| Rights: | Atribución-NoComercial-SinDerivadas 3.0 España ; http://creativecommons.org/licenses/by-nc-nd/3.0/es/ ; openAccess |
| رقم الانضمام: | edsbas.684EB4E5 |
| قاعدة البيانات: | BASE |
| تدمد: | 10504648 |
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| DOI: | 10.1016/j.fsi.2017.12.027 |