Academic Journal
Isolation of Primary Fibroblast Culture from Wildlife: the Panthera onca Case to Preserve a South American Endangered Species
| العنوان: | Isolation of Primary Fibroblast Culture from Wildlife: the Panthera onca Case to Preserve a South American Endangered Species |
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| المؤلفون: | Mestre‐Citrinovitz, Ana Cecilia, Sestelo, Adrián Jorge, Ceballos, María Belén, Barañao, José Lino, Saragüeta, Patricia |
| المصدر: | Current Protocols in Molecular Biology ; volume 116, issue 1 ; ISSN 1934-3639 1934-3647 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2016 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult‐to‐find, endangered and giant species like Jaguar ( Panthera onca ), the largest South American felid. This unit focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the Genetic Bank of Buenos Aires Zoo with the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species. © 2016 by John Wiley & Sons, Inc. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/cpmb.25 |
| الاتاحة: | http://dx.doi.org/10.1002/cpmb.25 http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcpmb.25 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpmb.25 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cpmb.25 https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpmb.25 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.682433B1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/cpmb.25 |
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