التفاصيل البيبلوغرافية
| العنوان: |
Lipid droplets are present in HSHA brain HPF at both 6 and 18 months of age. |
| المؤلفون: |
Virginie Lam (721740), Ryusuke Takechi (91201), Mark J. Hackett (1334373), Roslyn Francis (11432687), Michael Bynevelt (5576972), Liesl M. Celliers (11432690), Michael Nesbit (6483107), Somayra Mamsa (11432693), Frank Arfuso (696005), Sukanya Das (2638699), Frank Koentgen (4675591), Maree Hagan (11432696), Lincoln Codd (11432699), Kirsty Richardson (239689), Brenton O’Mara (11432702), Rainer K. Scharli (11432705), Laurence Morandeau (11432708), Jonathan Gauntlett (11432711), Christopher Leatherday (11432714), Jan Boucek (11432717), John C. L. Mamo (10736799) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Neuroscience, Immunology, Science Policy, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, passive avoidance test, marked neurodegeneration concomitant, 6j mice engineered, specific human amyloid, human amyloid restricted, &# 223, ), synthesise human, amyloid beta, suggesting impairment, peripheral metabolism, parenchymal extravasation, neurovascular inflammation, mediated axis, hsha mice, genetic modification, dependent learning, capillary dysfunction, capillary axis, alzheimer disease, ad ) |
| الوصف: |
(A) The distribution of the average size of lipid droplets stained by Sudan IV lipid stain in the HPF of 6-month-old (blue) and 18-month-old (red) HSHA mice with their age-matched controls. Data are expressed as means; n = 4–8 mice with approximately 100 images analysed. Statistical significance was assessed with two-way ANOVA (**** p < 0.0001). (B) The Pearson correlation coefficient of the mean area occupied by lipid droplets and number of lipid droplets per cell in the HPF region in young and aged HSHA and WT controls ( n = 4–8). (C, G, K, O) Representative bright field coronal micrographs of the HPF stained with Sudan IV in 6-month-old and 18-month-old HSHA mice and their age-matched WT controls. (D, H, L, P) Higher magnification of the HPF indicates a greater abundance/size of lipid droplets in blood vessels of the HPF of 18-month-old HSHA mice when compared to younger HSHA mice and their age-matched controls; indicated by the yellow arrowheads. (E, I, M, Q) Sudan IV positive-stained cells in the hippocampal CA1 pyramidal neuronal layer are indicated in higher abundance and size in aged HSHA mice compared to younger HSHA mice and their respective controls. (F, J, N, R) An abundance of interneuronal lipid droplets in the hippocampal CA1 stratum radiatum and pyramidal layers can be seen in both strains of aged mice; however, the 18-month HSHA cohort of mice (R) exhibited larger lipid droplets when compared to (J). Yellow arrowheads indicate lipid droplets stained by Sudan IV; black scale bars represent 50 μm (D, E, H, I, L, M, P, Q); white scale bars 20 μm (F, J, N, R). (S) Representative immunomicrograph of apo B staining (cyan) in the HPF of 12-month-old HSHA mice; scale bar represents 200 μm. (T) Representative magnified immunomicrograph of apo B staining (cyan) in the HPF of 12-month-old HSHA mice; scale bar represents 20 μm. (U-X) FTIR spectroscopy analysis of hippocampal lipid accumulation in HSHA mice. Representative images of lipid abundance in 4- and 18-month-old WT controls (U, W) and HSHA (V, X) ... |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Lipid_droplets_are_present_in_HSHA_brain_HPF_at_both_6_and_18_months_of_age_/16619192 |
| DOI: |
10.1371/journal.pbio.3001358.g003 |
| الاتاحة: |
https://doi.org/10.1371/journal.pbio.3001358.g003 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.67FF8209 |
| قاعدة البيانات: |
BASE |