التفاصيل البيبلوغرافية
| العنوان: |
Antiseizure potential of the ancient Greek medicinal plant Helleborus odorus subsp. cyclophyllus and identification of its main active principles |
| المؤلفون: |
Brillatz, Théo, Jacmin, Maxime, Vougogiannopoulou, Konstantina, Petrakis, Eleftherios A., Kalpoutzakis, Eleftherios, Houriet, Joëlle, Pellissier, Léonie, Rutz, Adriano, Marcourt, Laurence, Ferreira Queiroz, Emerson, Crawford, Alexander D., Skaltsounis, Alexios-Leandros, Wolfender, Jean-Luc |
| المصدر: |
ISSN: 0378-8741 ; Journal of Ethnopharmacology, vol. 259 (2020) 112954. |
| سنة النشر: |
2020 |
| المجموعة: |
Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: |
info:eu-repo/classification/ddc/615, Helleborus odorus subsp. cyclophyllus, Ethnopharmacology, Epilepsy, Zebrafish, Ranunculaceae |
| الوصف: |
Ethnopharmacological relevance Ethnopharmacological data and ancient texts support the use of black hellebore (Helleborus odorus subsp. cyclophyllus, Ranunculaceae) for the management and treatment of epilepsy in ancient Greece. Aim of the study A pharmacological investigation of the root methanolic extract (RME) was conducted using the zebrafish epilepsy model to isolate and identify the compounds responsible for a potential antiseizure activity and to provide evidence of its historical use. In addition, a comprehensive metabolite profiling of this studied species was proposed. Materials and methods The roots were extracted by solvents of increasing polarity and root decoction (RDE) was also prepared. The extracts were evaluated for antiseizure activity using a larval zebrafish epilepsy model with pentylenetetrazole (PTZ)-induced seizures. The RME exhibited the highest antiseizure activity and was therefore selected for bioactivity-guided fractionation. Isolated compounds were fully characterized by NMR and high-resolution tandem mass spectrometry (HRMS/MS). The UHPLC-HRMS/MS analyses of the RME and RDE were used for dereplication and metabolite profiling. Results The RME showed 80% inhibition of PTZ-induced locomotor activity (300μg/ml). This extract was fractionated and resulted in the isolation of a new glucopyranosyl-deoxyribonolactone (1) and a new furostanol saponin derivative (2), as well as of 20-hydroxyecdysone (3), hellebrin (4), a spirostanol glycoside derivative (5) and deglucohellebrin (6). The antiseizure activity of RME was found to be mainly due to the new furostanol saponin (2) and hellebrin (4), which reduced 45% and 60% of PTZ-induced seizures (135μM, respectively). Besides, the aglycone of hellebrin, hellebrigenin (S34), was also active (45% at 7μM). To further characterize the chemical composition of both RME and RDE, 30 compounds (A7-33, A35–37) were annotated based on UHPLC-HRMS/MS metabolite profiling. This revealed the presence of additional bufadienolides, furostanols, and evidenced ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/32445663; info:eu-repo/semantics/dataset/url/https://doi.org/10.17632/nbbx2x83x6.2; https://archive-ouverte.unige.ch/unige:138886; unige:138886 |
| الاتاحة: |
https://archive-ouverte.unige.ch/unige:138886 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.679D0923 |
| قاعدة البيانات: |
BASE |