Academic Journal
Profiling the lncRNA-miRNA-mRNA interaction network in the submandibular gland of diabetic mice
| العنوان: | Profiling the lncRNA-miRNA-mRNA interaction network in the submandibular gland of diabetic mice |
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| المؤلفون: | Shi, Xi-Jin, Liu, Hui-Min, Li, Li, Zhang, Yan, Cong, Xin, Liu, Li-Mei, Wu, Li-ling, Xiang, Ruo-Lan |
| المساهمون: | the National Natural Science Foundation of China, Beijing Natural Science Foundation |
| المصدر: | BMC Endocrine Disorders ; volume 22, issue 1 ; ISSN 1472-6823 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| الوصف: | Background Hyposalivation is one of the common symptoms of diabetes. Although long non-coding RNAs (lncRNAs) have recently been reported to play important roles in the pathogenesis of diabetes, the role of lncRNAs in diabetes-induced hyposalivation remains unknown. Methods The present study aimed to explore the function of lncRNA-microRNA-mRNA regulatory network in the submandibular gland (SMGs) under the context of diabetes. LncRNA expression profile of the SMGs was analyzed using microarray technology. Differentially expressed lncRNAs were confirmed using real-time quantitative PCR. Bioinformatics analyses were performed, and Coding-non-coding gene co-expression (CNC) and competing endogenous RNA (ceRNA) networks were constructed to explore the potential mechanisms of diabetes-induced hyposalivation. Results A total of 1273 differentially expressed lncRNAs (536 up-regulated and 737 downregulated) were identified in the SMGs tissues of db/db mice. CNC and ceRNA network analyses were performed based on five differentially expressed lncRNAs validated by real-time quantitative PCR. Gene Ontology analysis of target genes of CNC network revealed that “calcium ion binding” was a highly enriched molecular function. Kyoto Encyclopedia of Genes and Genomes pathway analysis of target genes of ceRNA network revealed that the “mammalian target of rapamycin signaling pathway” was significantly enriched. Conclusions On the whole, the findings of the present study may provide insight into the possible mechanism of diabetes-induced hyposalivation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s12902-022-01019-1 |
| DOI: | 10.1186/s12902-022-01019-1.pdf |
| DOI: | 10.1186/s12902-022-01019-1/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s12902-022-01019-1 https://link.springer.com/content/pdf/10.1186/s12902-022-01019-1.pdf https://link.springer.com/article/10.1186/s12902-022-01019-1/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.678BFAE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12902-022-01019-1 |
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