Academic Journal
Boosting innate immunity: Asaia bacteria expressing a protein from Wolbachia determine macrophage activation and killing of Leishmania
| العنوان: | Boosting innate immunity: Asaia bacteria expressing a protein from Wolbachia determine macrophage activation and killing of Leishmania |
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| المؤلفون: | ILARIA VAROTTO-BOCCAZZI, SARA EPIS, IRENE ARNOLDI, YOLANDA CORBETT, PAOLO GABRIELI, MOIRA PARONI, RICCARDO NODARI, NICOLETTA BASILICO, LUCIANO SACCHI, MARINA GRAMICCIA, LUIGI GRADONI, VITO TRANQUILLO, Claudio Bandi |
| المساهمون: | I. VAROTTO BOCCAZZI, S. Epi, I. Arnoldi, Y. Corbett, P. Gabrieli, M. Paroni, R. Nodari, N. Basilico, L. Sacchi, M. Gramiccia, L. Gradoni, V. Tranquillo, C. Bandi |
| سنة النشر: | 2020 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | symbionts, vaccine vehicles, filarial nematode, Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali, Settore BIO/19 - Microbiologia Generale |
| الوصف: | Leishmaniases are severe vector-borne diseases affecting humans and animals, caused by Leishmania protozoans. Immune polarization plays a major role in determining the outcome of Leishmania infections: hosts displaying M1-polarized macrophages are protected, while those biased on the M2 side acquire a chronic infection, that could develop into an overt and potentially deadly disease. The identification of the factors involved in M1 polarization is essential for the design of therapeutic and prophylactic interventions, including vaccines. Infection by the filarial nematode Dirofilaria immitis could be one of the factors that interfere with leishmaniasis in dogs. Indeed, filarial nematodes induce a partial skew of the immune response towards M1, likely caused by their bacterial endosymbionts, Wolbachia. Here we have examined the potential of AsaiaWSP, a bacterium engineered for the expression of the Wolbachia surface protein (WSP), as an inductor of M1 macrophage activation and Leishmania killing. Macrophages stimulated with AsaiaWSP displayed a strong leishmanicidal activity, comparable to that determined by the choice-drug amphotericin B. Additionally, AsaiaWSP determined the expression of markers of classical macrophage activation, including M1 cytokines, ROS and NO, and an increase in phagocytosis activity. Asaia not expressing WSP also induced macrophage activation, although at a lower extent compared to AsaiaWSP. In summary, our study, while providing a strong evidence for the immune-stimulating properties of Wolbachia, highlights the translational potential of AsaiaWSP in the areas of the immune-prophylaxis and therapy of leishmaniases, as well as of other diseases that could be subverted by M1 macrophage activation. Competing Interest Statement The authors have declared no competing interest. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | http://hdl.handle.net/2434/878402; https://www.biorxiv.org/content/10.1101/2020.06.22.164145v2 |
| DOI: | 10.1101/2020.06.22.164145 |
| DOI: | 10.1101/2020.06.22.164145v2 |
| الاتاحة: | http://hdl.handle.net/2434/878402 https://doi.org/10.1101/2020.06.22.164145 https://www.biorxiv.org/content/10.1101/2020.06.22.164145v2 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.67721A1E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2020.06.22.164145 |
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