التفاصيل البيبلوغرافية
| العنوان: |
Inflammatory reactivity as a marker of the potential health effects of Dense Non-Aqueous Phase Liquids in contaminated groundwater |
| المؤلفون: |
Tshitenge, Christine Tshibuayi |
| سنة النشر: |
2012 |
| المجموعة: |
The University of Johannesburg: UJContent |
| مصطلحات موضوعية: |
Groundwater pollution, Groundwater quality measurement, Dense non-aqueous phase liquids |
| الوصف: |
M.Tech. ; Background: Contaminated groundwater threatens many valuable water resources with potentially disastrous consequences. Because of the non-visible nature of groundwater, water testing methods are required that can detect not only the occurrence of dense non-aqueous phase liquids (DNAPL)-like substances in groundwater, but also address human health-related effects of the water upon consumption. Assuming ingestion of DNAPL-contaminated water may, depending on the concentration, mediate an inflammatory response or cause cellular injuries in the human body, like any other contaminant in water, which could compromise the immune response and cause numerous health problems. Hence the aim of the study was to develop and test methodologies from known immunological-testing techniques to establish whether DNAPL-contaminated drinking water would cause health-related effects. Objectives: To develop a DNAPL reference model based on positive and baseline controls:(1) Using known concentrations of index chemical from each of two DNAPL groups i.e. trichloroethylene (TCE) from the chlorinated solvents (CS) and Aroclor®1254 (ARO) from polychlorinated biphenyls (PCB), to expose PBMC and test the reactions based on Apoptosis, Nitric oxide production and Cytokine expression (ANOPCE); (2) Using non-contaminated ground- and other treated waters suitable for drinking with ANOPCE; (3) Applying the ANOPCE-based method to various groundwater samples taken from a contaminated industrial site. Methods: The method focussed on measuring in vitro immune responses of peripheral blood mononuclear cells harvested from human whole-blood. The responses measured were 1) Apoptosis (programmed cell death), and 2) inflammation characterised by increased nitric oxide production (NOP) and cytokine expression (CE). Results: The following results were obtained: Index chemical Time study: TCE increased cell viability and NOP in a time-dependent manner and ARO lead to increased IL-1β and Apoptotic cell death; Index chemical Concentration study: ... |
| نوع الوثيقة: |
thesis |
| اللغة: |
unknown |
| Relation: |
uj:2214; http://hdl.handle.net/10210/4607 |
| الاتاحة: |
http://hdl.handle.net/10210/4607 |
| رقم الانضمام: |
edsbas.67692B54 |
| قاعدة البيانات: |
BASE |