Academic Journal
Application of efficient and specific gene transfer systems and organ culture techniques for the elucidation of mechanisms of epithelial– mesenchymal interaction in the developing gut
| العنوان: | Application of efficient and specific gene transfer systems and organ culture techniques for the elucidation of mechanisms of epithelial– mesenchymal interaction in the developing gut |
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| المؤلفون: | Fukuda, Kimiko, Sakamoto, Nobuyuki, Narita, Tomohiro, Saitoh, Kanako, Kameda, Takashi, Iba, Hideo, Yasugi, Sadao |
| المصدر: | Development, Growth & Differentiation ; volume 42, issue 3, page 207-211 ; ISSN 0012-1592 1440-169X |
| بيانات النشر: | Wiley |
| سنة النشر: | 2000 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Epithelial–mesenchymal interactions are very important in the development of the vertebrate gut. In the avian embryonic stomach (proventriculus), expression of embryonic chick pepsinogen (ECPg) gene, which is specific to developing glandular cells in stomach epithelium, is regulated by mesenchymal influence. Molecular mechanisms of tissue‐specific transcriptional regulation of the ECPg gene and the molecular nature of the mesenchymal signals were analyzed using a combination of the classic organ culture system and gene transfer strategies. In the present review, three methods for the introduction of DNA into tissues are described: lipofection, electroporation and retroviral infection, and characteristics of each system are discussed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1046/j.1440-169x.2000.00503.x |
| الاتاحة: | http://dx.doi.org/10.1046/j.1440-169x.2000.00503.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1440-169x.2000.00503.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1440-169x.2000.00503.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.66FD15E2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1046/j.1440-169x.2000.00503.x |
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