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Aurélie Francois,1,2 Luca Dirheimer,1,2 Alicia Chateau,2 Henri-Pierre Lassalle,1,2 Ilya Yakavets,3 Lina Bezdetnaya1,2 1Research Department, Institut de Cancérologie de Lorraine, Vandoeuvre-lès-Nancy, France; 2Centre de Recherche en Automatique de Nancy, Centre National de la Recherche Scientifique, UMR 7039, Université de Lorraine, Vandoeuvre-lès-Nancy, France; 3Department of Chemistry, University of Toronto, Toronto, CanadaCorrespondence: Lina Bezdetnaya, Research Department, Institut de Cancérologie de Lorraine, 6 avenue de Bourgogne, Vandoeuvre-lès-Nancy, 54519, France, Tel +33-03-8359-8353, Email l.bolotine@nancy.unicancer.frPurpose: The tumor microenvironment (TME) is composed of various stromal components, including immune cells such as tumor-associated macrophages (TAMs), which play a crucial role in cancer initiation and progression. TAMs can exhibit either a tumor-suppressive M1 or a tumor-promoting M2 phenotype. First, we aimed to develop a 3D human heterotypic model consisting of head and neck squamous cell carcinoma (HNSCC) cells and different subtypes of macrophages to replicate the interactions between immune cells and cancer cells. We further investigated the behavior of Foslip®, a liposomal formulation of temoporfin, using a macrophage-enriched 3D model.Methods: Monocytes were differentiated into M1 and M2 macrophages, which represent two distinct subtypes. Following histological and molecular characterization, these macrophages were used to establish a 3D spheroid model of HNSCC enriched with either polarized macrophages or conditioned media. Flow cytometry and fluorescence microscopy were used to assess the accumulation and distribution of Foslip®. The cytotoxic effect of Foslip®-mediated photodynamic therapy (PDT) was evaluated using flow cytometry.Results: We developed heterotypic spheroids characterized by a mixed phenotype of evenly distributed macrophages. In this 3D co-culture model, both M1 and M2 macrophages showed significantly higher accumulation of Foslip® compared to the ... |