التفاصيل البيبلوغرافية
| العنوان: |
Genome-Wide Identification of GmSPS Gene Family in Soybean and Expression Analysis in Response to Cold Stress |
| المؤلفون: |
Jiafang Shen, Yiran Xu, Songli Yuan, Fuxiao Jin, Yi Huang, Haifeng Chen, Zhihui Shan, Zhonglu Yang, Shuilian Chen, Xinan Zhou, Chanjuan Zhang |
| المصدر: |
International Journal of Molecular Sciences; Volume 24; Issue 16; Pages: 12878 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2023 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
soybean, sucrose phosphate synthase, genome-wide survey, gene expression, cold stress |
| جغرافية الموضوع: |
agris |
| الوصف: |
Sucrose metabolism plays a critical role in development, stress response, and yield formation of plants. Sucrose phosphate synthase (SPS) is the key rate-limiting enzyme in the sucrose synthesis pathway. To date, genome-wide survey and comprehensive analysis of the SPS gene family in soybean (Glycine max) have yet to be performed. In this study, seven genes encoding SPS were identified in soybean genome. The structural characteristics, phylogenetics, tissue expression patterns, and cold stress response of these GmSPSs were investigated. A comparative phylogenetic analysis of SPS proteins in soybean, Medicago truncatula, Medicago sativa, Lotus japonicus, Arabidopsis, and rice revealed four families. GmSPSs were clustered into three families from A to C, and have undergone five segmental duplication events under purifying selection. All GmSPS genes had various expression patterns in different tissues, and family A members GmSPS13/17 were highly expressed in nodules. Remarkably, all GmSPS promoters contain multiple low-temperature-responsive elements such as potential binding sites of inducer of CBF expression 1 (ICE1), the central regulator in cold response. qRT-PCR proved that these GmSPS genes, especially GmSPS8/18, were induced by cold treatment in soybean leaves, and the expression pattern of GmICE1 under cold treatment was similar to that of GmSPS8/18. Further transient expression analysis in Nicotiana benthamiana and electrophoretic mobility shift assay (EMSA) indicated that GmSPS8 and GmSPS18 transcriptions were directly activated by GmICE1. Taken together, our findings may aid in future efforts to clarify the potential roles of GmSPS genes in response to cold stress in soybean. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Molecular Plant Sciences; https://dx.doi.org/10.3390/ijms241612878 |
| DOI: |
10.3390/ijms241612878 |
| الاتاحة: |
https://doi.org/10.3390/ijms241612878 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.66EB4F73 |
| قاعدة البيانات: |
BASE |