Academic Journal
Regulation of the positive transcriptional effect of PLZF through a non-canonical EZH2 activity
| العنوان: | Regulation of the positive transcriptional effect of PLZF through a non-canonical EZH2 activity |
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| المؤلفون: | Koubi, Myriam, Poplineau, Mathilde, Vernerey, Julien, N 'Guyen, Lia, Tiberi, Guillaume, Garciaz, Sylvain, El-Kaoutari, Abdessamad, Maqbool, Muhammad, A, Andrau, Jean-Christophe, Guillouf, Christel, Saurin, Andrew, J, Duprez, Estelle |
| المساهمون: | Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes (IPC), Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Hématopoïèse normale et pathologique (U1170 Inserm), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris-Saclay, Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0305-1048. |
| بيانات النشر: | HAL CCSD Oxford University Press |
| سنة النشر: | 2018 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.CAN]Life Sciences [q-bio]/Cancer |
| الوصف: | International audience ; The transcription factor PLZF (promyelocytic leukemia zinc finger protein) acts as an epigenetic regulator balancing self-renewal and differentiation of hematopoietic cells through binding to various chromatin-modifying factors. First described as a transcriptional repressor, PLZF is also associated with active transcription, although the molecular bases underlying the differences are unknown. Here, we reveal that in a hematopoietic cell line, PLZF is predominantly associated with transcribed genes. Additionally, we identify a new association between PLZF and the histone methyltransferase, EZH2 at the genomic level. We find that co-occupancy of PLZF and EZH2 on chromatin at PLZF target genes is not associated with SUZ12 or trimethylated lysine 27 of histone H3 (H3K27me3) but with the active histone mark H3K4me3 and active transcription. Removal of EZH2 leads to an increase of PLZF binding and increased gene expression. Our results suggest a new role of EZH2 in restricting PLZF positive transcriptional activity independently of its canonical PRC2 activity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/nar/gky080 |
| الاتاحة: | https://hal.science/hal-01765664 https://hal.science/hal-01765664v1/document https://hal.science/hal-01765664v1/file/gky080.pdf https://doi.org/10.1093/nar/gky080 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.66ABE7C4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gky080 |
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