Academic Journal
The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken
| العنوان: | The seminal acrosin‐inhibitor ClTI1/SPINK2 is a fertility‐associated marker in the chicken |
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| المؤلفون: | Thelie, Aurore, Réhault-Godbert, Sophie, Poirier, Jean-Claude, Govoroun, Marina, Fouchécourt, Sophie, Blesbois, Elisabeth |
| المساهمون: | Physiologie de la reproduction et des comportements Nouzilly (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation Saumur (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Biologie des Oiseaux et Aviculture (BOA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) |
| المصدر: | ISSN: 1040-452X. |
| بيانات النشر: | HAL CCSD Wiley |
| سنة النشر: | 2019 |
| المجموعة: | Université de Poitiers: Publications de nos chercheurs.ses (HAL) |
| مصطلحات موضوعية: | acrosin, chicken, male fertility, protease inhibitor, seminal plasma, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology |
| الوصف: | International audience ; The seminal plasma is a very complex fluid, which surrounds sperm in semen. It contains numerous proteins including proteases and protease inhibitors that regulate proteolytic processes associated with protein activation and degradation. We previously identified a seminal protein, chicken liver trypsin inhibitor 1 (ClTI‐1) over expressed in semen of roosters with high fertility, suggesting a role in male fertility. In the present study, we showed that ClTI‐1 gene is actually SPINK2. Using normal healthy adult roosters, we showed that SPINK2 amount in seminal plasma was positively correlated with male fertility in chicken lines with highly contrasted genetic backgrounds (broiler and layer lines). Using affinity chromatography combined to mass spectrometry analysis and kinetic assays, we demonstrated for the first time that two chicken acrosin isoforms (acrosin and acrosin‐like proteins) are the physiological serine protease targets of SPINK2 inhibitor. SPINK2 transcript was overexpressed all along the male tract, and the protein was present in the lumen as expected for secreted proteins. Altogether, these data emphasize the role of seminal SPINK2 Kazal‐type inhibitor as an important actor of fertility in birds through its inhibitory action on acrosin isoforms proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-475960-9 0-00-475960-5 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/31033055; hal-02620572; https://hal.inrae.fr/hal-02620572; https://hal.inrae.fr/hal-02620572/document; https://hal.inrae.fr/hal-02620572/file/Thelie%20et%20al_1.2019; PRODINRA: 469353; PUBMED: 31033055; WOS: 000475960500002 |
| DOI: | 10.1002/mrd.23153 |
| الاتاحة: | https://hal.inrae.fr/hal-02620572 https://hal.inrae.fr/hal-02620572/document https://hal.inrae.fr/hal-02620572/file/Thelie%20et%20al_1.2019 https://doi.org/10.1002/mrd.23153 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.6698D123 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780004759609 0004759605 |
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| DOI: | 10.1002/mrd.23153 |