Academic Journal
Functional expression of the Na/K pump is controlled via a cyclosporin A‐sensitive signalling pathway in activated human lymphocytes
| العنوان: | Functional expression of the Na/K pump is controlled via a cyclosporin A‐sensitive signalling pathway in activated human lymphocytes |
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| المؤلفون: | Marakhova, I.I, Ivanova, A.E, Toropova, F.V, Vereninov, A.A, Vinogradova, T.A |
| المصدر: | FEBS Letters ; volume 456, issue 2, page 285-289 ; ISSN 0014-5793 1873-3468 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1999 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | An immunosuppressant cyclosporin A (CsA) inhibits T‐cell proliferation by blocking the nuclear factor of activated T‐cells (NFAT) required for expression of the interleukin‐2 (IL‐2) gene. This work has demonstrated for the first time that in human blood lymphocytes (HBLs) activated by phytohemagglutinin (PHA), CsA at anti‐proliferative doses inhibits the late sustained increase in ouabain‐sensitive Rb(K) influxes, which accompanies the growth phase of G 0 /G 1 /S transition. CsA affects neither the initial, transient activation of the pump in response to PHA nor the ouabain‐resistant ion fluxes during cell cycle progression. When the HBLs were rendered competent to proliferate by phorbol 12,13‐dibutyrate ester and ionomycin in the presence of CsA, the exogenous IL‐2 did not bypass the initial inhibitory effect of CsA on the long‐term pump enhancement. When applied after the competence induction, CsA produced no effect on the sustained increase in ouabain‐sensitive Rb influxes during the IL‐2‐induced progression phase. These results indicate that in activated HBLs, (1) IL‐2 is involved in functional expression of the Na/K pump during cell transition from quiescence to proliferation, (2) the cell cycle‐associated upregulation of the pump is related to a CsA‐sensitive signalling pathway. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/s0014-5793(99)00955-2 |
| DOI: | 10.1016/S0014-5793%2899%2900955-2 |
| الاتاحة: | http://dx.doi.org/10.1016/s0014-5793(99)00955-2 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2899%2900955-2 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(99)00955-2 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2899%2900955-2 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.664C8369 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/s0014-5793(99)00955-2 |
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