Academic Journal
The transcriptionally active regions in the genome of Bacillus subtilis
| العنوان: | The transcriptionally active regions in the genome of Bacillus subtilis |
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| المؤلفون: | Rasmussen, Simon, Nielsen, Henrik Bjørn, Jarmer, Hanne |
| المصدر: | Molecular Microbiology ; volume 73, issue 6, page 1043-1057 ; ISSN 0950-382X 1365-2958 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2009 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Summary The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand‐specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome‐wide expression during mid‐exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non‐coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22 , is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA . Through this work we have discovered a long conserved 3′ untranslated region (UTR) in a group of membrane‐associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN , which encodes a target of the twin‐arginine translocase (Tat) protein translocation system. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1365-2958.2009.06830.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1365-2958.2009.06830.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2958.2009.06830.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.2009.06830.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.66407C58 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-2958.2009.06830.x |
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