Academic Journal
RNA-sequencing of paired tape-strips and skin biopsies in atopic dermatitis reveals key differences
| العنوان: | RNA-sequencing of paired tape-strips and skin biopsies in atopic dermatitis reveals key differences |
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| المؤلفون: | Fritz, Blaine, Halling, Anne Sofie, Cort, Isabel Díaz Pinés, Christensen, Maria Oberländer, Rønnstad, Amalie Thorsti Møller, Olesen, Caroline Meyer, Knudgaard, Mette Hjorslev, Zachariae, Claus, Heegaard, Steffen, Thyssen, Jacob P., Bjarnsholt, Thomas |
| المصدر: | Fritz , B , Halling , A S , Cort , I D P , Christensen , M O , Rønnstad , A T M , Olesen , C M , Knudgaard , M H , Zachariae , C , Heegaard , S , Thyssen , J P & Bjarnsholt , T 2024 , ' RNA-sequencing of paired tape-strips and skin biopsies in atopic dermatitis reveals key differences ' , Allergy: European Journal of Allergy and Clinical Immunology , pp. 1548-1559 . https://doi.org/10.1111/all.16086 |
| سنة النشر: | 2024 |
| المجموعة: | University of Copenhagen: Research / Forskning ved Københavns Universitet |
| مصطلحات موضوعية: | atopic dermatitis, biopsies, inflammation, RNA sequencing, tape-strips |
| الوصف: | Background Skin tape-strips and biopsies are widely used methods for investigating the skin in atopic dermatitis (AD). Biopsies are more commonly used but can cause scarring and pain, whereas tape-strips are noninvasive but sample less tissue. The study evaluated the performance of skin tape-strips and biopsies for studying AD. Methods Whole-transcriptome RNA-sequencing was performed on paired tape-strips and biopsies collected from lesional and non-lesional skin from AD patients (n = 7) and non-AD controls (n = 5). RNA yield, mapping efficiency, and differentially expressed genes (DEGs) for the two methods (tape-strip/biopsy) and presence of AD (AD/non-AD) were compared. Results Tape-strips demonstrated a lower RNA yield (22 vs. 4596 ng) and mapping efficiency to known genes (28% vs. 93%) than biopsies. Gene-expression profiles of paired tape-strips and biopsies demonstrated a medium correlation (R2 = 0.431). Tape-strips and biopsies demonstrated systematic differences in measured expression levels of 6483 genes across both AD and non-AD samples. Tape-strips preferentially detected many itch (CCL3/CCL4/OSM) and immune-response (CXCL8/IL4/IL5/IL22) genes as well as markers of epidermal dendritic cells (CD1a/CD207), while certain cytokines (IL18/IL37), skin-barrier genes (KRT2/FLG2), and dermal fibroblasts markers (COL1A/COL3A) were preferentially detected by biopsies. Tape-strips identified more DEGs between AD and non-AD (3157 DEGs) then biopsies (44 DEGs). Tape-strips also detected higher levels of bacterial mRNA than biopsies. Conclusions This study concludes that tape-strips and biopsies each demonstrate respective advantages for measuring gene-expression changes in AD. Thus, the specific skin layers and genes of interest should be considered before selecting either method. ; Background: Skin tape-strips and biopsies are widely used methods for investigating the skin in atopic dermatitis (AD). Biopsies are more commonly used but can cause scarring and pain, whereas tape-strips are noninvasive but sample less ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1111/all.16086 |
| الاتاحة: | https://researchprofiles.ku.dk/da/publications/rnasequencing-of-paired-tapestrips-and-skin-biopsies-in-atopic-dermatitis-reveals-key-differences(b1f6cdbc-88f2-406e-b40f-f1612ad14c46).html https://doi.org/10.1111/all.16086 https://curis.ku.dk/ws/files/393263322/RNA_sequencing.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.661D9D7A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/all.16086 |
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