التفاصيل البيبلوغرافية
| العنوان: |
DataSheet_1_Disparate Interferon Signaling and Shared Aberrant Basaloid Cells in Single-Cell Profiling of Idiopathic Pulmonary Fibrosis and Systemic Sclerosis-Associated Interstitial Lung Disease.docx |
| المؤلفون: |
Eleanor Valenzi (8508294), Tracy Tabib (1874692), Anna Papazoglou (3628385), John Sembrat (2657644), Humberto E. Trejo Bittar (10495430), Mauricio Rojas (173910), Robert Lafyatis (38139) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, systemic sclerosis, systemic sclerosis (scleroderma), idiopathic pulmonary fibrosis, interstitial lung disease (ILD), single-cell RNA-sequencing (scRNA-seq) |
| الوصف: |
Idiopathic pulmonary fibrosis (IPF) and systemic sclerosis-associated interstitial lung disease (SSc-ILD) differ in the predominant demographics and identified genetic risk alleles of effected patients, however both diseases frequently progress to respiratory failure and death. Contrasting advanced SSc-ILD to IPF provides insight to the role dysregulated immunity may play in pulmonary fibrosis. To analyze cell-type specific transcriptome commonalities and differences between IPF and SSc-ILD, we compared single-cell RNA-sequencing (scRNA-seq) of 21 explanted lung tissue specimens from patients with advanced IPF, SSc-ILD, and organ donor controls. Comparison of IPF and SSc-ILD tissue identified divergent patterns of interferon signaling, with interferon-gamma signaling upregulated in the SPP1 hi and FABP4 hi macrophages, cytotoxic T cells, and natural kill cells of IPF, while type I interferon signaling and production was upregulated in the corresponding SSc-ILD populations. Plasmacytoid dendritic cells were found in diseased lungs only, and exhibited upregulated cellular stress pathways in SSc-ILD compared to IPF. Alveolar type I cells were dramatically decreased in both IPF and SSc-ILD, with a distinct transcriptome signature separating these cells by disease. KRT5 - /KRT17 + aberrant basaloid cells exhibiting markers of cellular senescence and epithelial-mesenchymal transition were identified in SSc-ILD for the first time. In summary, our study utilizes the enriched capabilities of scRNA-seq to identify key divergent cell types and pathways between IPF and SSc-ILD, providing new insights into the shared and distinct mechanisms between idiopathic and autoimmune interstitial lung diseases. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/DataSheet_1_Disparate_Interferon_Signaling_and_Shared_Aberrant_Basaloid_Cells_in_Single-Cell_Profiling_of_Idiopathic_Pulmonary_Fibrosis_and_Systemic_Sclerosis-Associated_Interstitial_Lung_Disease_docx/14338196 |
| DOI: |
10.3389/fimmu.2021.595811.s001 |
| الاتاحة: |
https://doi.org/10.3389/fimmu.2021.595811.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.65BCF28F |
| قاعدة البيانات: |
BASE |