التفاصيل البيبلوغرافية
| العنوان: |
Cellulose Biosynthesis Inhibitors: Comparative Effect on Bean Cell Cultures |
| المؤلفون: |
Penélope García-Angulo, Ana Alonso-Simón, Antonio Encina, Jesús M. Álvarez, José L. Acebes |
| المصدر: |
International Journal of Molecular Sciences; Volume 13; Issue 3; Pages: 3685-3702 |
| بيانات النشر: |
Molecular Diversity Preservation International |
| سنة النشر: |
2012 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
AE F150944, cell wall, cell cultures, cellulose biosynthesis-inhibitor (CBI) herbicides, CGA 325′615, compound 1, dichlobenil, Phaseolus vulgaris L., quinclorac, triazofenamide |
| جغرافية الموضوع: |
agris |
| الوصف: |
The variety of bioassays developed to evaluate different inhibition responses for cellulose biosynthesis inhibitors makes it difficult to compare the results obtained. This work aims (i) to test a single inhibitory assay for comparing active concentrations of a set of putative cellulose biosynthesis inhibitors and (ii) to characterize their effect on cell wall polysaccharides biosynthesis following a short-term exposure. For the first aim, dose-response curves for inhibition of dry-weight increase following a 30 days exposure of bean callus-cultured cells to these inhibitors were obtained. The compound concentration capable of inhibiting dry weight increase by 50% compared to control (I50) ranged from subnanomolar (CGA 325′615) to nanomolar (AE F150944, flupoxam, triazofenamide and oxaziclomefone) and micromolar (dichlobenil, quinclorac and compound 1) concentrations. In order to gain a better understanding of the effect of the putative inhibitors on cell wall polysaccharides biosynthesis, the [14C]glucose incorporation into cell wall fractions was determined after a 20 h exposure of cell suspensions to each inhibitor at their I50 value. All the inhibitors tested decreased glucose incorporation into cellulose with the exception of quinclorac, which increased it. In some herbicide treatments, reduction in the incorporation into cellulose was accompanied by an increase in the incorporation into other fractions. In order to appreciate the effect of the inhibitors on cell wall partitioning, a cluster and Principal Component Analysis (PCA) based on the relative contribution of [14C]glucose incorporation into the different cell wall fractions were performed, and three groups of compounds were identified. The first group included quinclorac, which increased glucose incorporation into cellulose; the second group consisted of compound 1, CGA 325′615, oxaziclomefone and AE F150944, which decreased the relative glucose incorporation into cellulose but increased it into tightly-bound cellulose fractions; and the third ... |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://dx.doi.org/10.3390/ijms13033685 |
| DOI: |
10.3390/ijms13033685 |
| الاتاحة: |
https://doi.org/10.3390/ijms13033685 |
| Rights: |
https://creativecommons.org/licenses/by/3.0/ |
| رقم الانضمام: |
edsbas.65B0290 |
| قاعدة البيانات: |
BASE |