Academic Journal
The New Tumour Biomarker miRNA‐371‐3p Influences Cisplatin Sensitivity of Testicular Germ Cell Tumour Cell Lines
| العنوان: | The New Tumour Biomarker miRNA‐371‐3p Influences Cisplatin Sensitivity of Testicular Germ Cell Tumour Cell Lines |
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| المؤلفون: | Weiten, Richard, Engler, Theadora, Schorle, Hubert, Ellinger, Jörg, Saponaro, Miriam, Alajati, Abdullah, Nettersheim, Daniel, Syring‐Schmandke, Isabella |
| المصدر: | Journal of Cellular and Molecular Medicine ; volume 28, issue 24 ; ISSN 1582-1838 1582-4934 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2024 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Cisplatin is used to treat a variety of malignancies, including testicular germ cell tumours (TGCTs). Although cisplatin‐based chemotherapy yields high response rates, a subset of patients develop cisplatin resistance, limiting treatment options and worsening prognosis. Therefore, there is a high clinical need for new therapeutic strategies targeting cisplatin‐resistant TGCTs. MicroRNA‐371a‐3p ( miR‐371 ), the new serum biomarker for TGCTs, shows significantly increased expression in cisplatin‐resistant TGCT cell lines compared to sensitive parental cell lines. However, the functional impact of miR‐371 on cisplatin sensitivity has not been investigated yet. To evaluate the impact of miR‐37 1 on cisplatin sensitivity, antagomirs were used to inhibit miR‐371 expression, resulting in a > 98% decrease in miR‐371 expression. Cisplatin sensitivity was significantly increased after miR‐371 inhibition in cisplatin‐resistant and corresponding parental TGCT cell lines, indicating a strongly reduced viability and increased apoptosis after cisplatin treatment in miR‐371 ‐inhibited cells. Our results suggest that miR‐371 may contribute to the development of cisplatin resistance in TGCTs. Interfering with miR‐371 expression can increase the cisplatin sensitivity of tumour cells, which may represent a promising approach to improve future therapeutic outcomes in patients with TGCTs, especially those with cisplatin‐resistant disease. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/jcmm.70314 |
| الاتاحة: | https://doi.org/10.1111/jcmm.70314 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jcmm.70314 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.6558D4A4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/jcmm.70314 |
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