Academic Journal
Role of autofluorescence technique in detection of mycobacterial bacilli on fine needle aspiration cytology in tubercular lymphadenitis in comparison to conventional methods
| العنوان: | Role of autofluorescence technique in detection of mycobacterial bacilli on fine needle aspiration cytology in tubercular lymphadenitis in comparison to conventional methods |
|---|---|
| المؤلفون: | Kumar, Sanjay, Gupta, Ritika, Kaushik, Nidhi, Kataria, Sant Prakash, Singh, Sunita |
| المصدر: | International Journal of Research in Medical Sciences; Vol 10, No 4 (2022): April 2022; 926-932 ; 2320-6012 ; 2320-6071 |
| بيانات النشر: | Medip Academy |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Tuberculosis, Autofluorescence, FNAC, Lymph node |
| الوصف: | Background: Lymphadenopathy is the most common presentation of extrapulmonary tuberculosis. Fine needle aspiration cytology (FNAC) has emerged an important role in the evaluation of peripheral adenopathy as a possible non-invasive procedure. Conventional Ziehl-Neelsen (ZN) method for acid fast bacilli (AFB) plays an important role in the diagnosis and the monitoring of treatment in tuberculosis. Fluorescence microscopy using auramine-rhodamine (AR) or Papanicolaou (PAP) staining has been considered superior to ZN staining.Methods: The study was conducted in tertiary care centre for a period of 2 years. A total number of 75 patients were included in the study. Four smears made from each aspirate: three air dried smears were stained with Giemsa, ZN, and AR stains and one was wet fixed for PAP stain for autofluorescence. Aspirate sent for culture over Lowenstein-Jensen medium was taken as a reference method.Results: Seventy five aspirates reported as tuberculous lymphadenitis on cytomorphology were stained with various methods. Autofluorescence was of more diagnostic utility for detection of AFB when cytologically only necrosis (64.71%) or granulomatous lymphadenitis (42.11%) was seen. With necrotising granulomatous lymphadenitis reported cases, Auramine Rhodamine was positive in maximum cases (66.7%) and was significant statistically. Overall taking culture as gold standard ZN stain was most specific (100%) and Auramine Rhodamine was most sensitive (82%).Conclusions: There is a definite advantage of autofluorescence in detecting mycobacteria over Ziehl-Neelsen and auramine rhodamine stain as it is more sensitive as well as an inexpensive technique. Autofluorescence can be a useful addition to routine cytology for early diagnosis and effective treatment. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://www.msjonline.org/index.php/ijrms/article/view/10215/7066; https://www.msjonline.org/index.php/ijrms/article/downloadSuppFile/10215/10364; https://www.msjonline.org/index.php/ijrms/article/downloadSuppFile/10215/10365; https://www.msjonline.org/index.php/ijrms/article/view/10215 |
| DOI: | 10.18203/2320-6012.ijrms20220987 |
| الاتاحة: | https://www.msjonline.org/index.php/ijrms/article/view/10215 https://doi.org/10.18203/2320-6012.ijrms20220987 |
| Rights: | Copyright (c) 2022 International Journal of Research in Medical Sciences |
| رقم الانضمام: | edsbas.6542820B |
| قاعدة البيانات: | BASE |
| DOI: | 10.18203/2320-6012.ijrms20220987 |
|---|