التفاصيل البيبلوغرافية
| العنوان: |
Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice |
| المؤلفون: |
Sakai, Yusuke, Yamanouchi, Kosho, Ohashi, Kazuo, Koike, Makiko, Utoh, Rie, Hasegawa, Hideko, Muraoka, Izumi, Suematsu, Takashi, Soyama, Akihiko, Hidaka, Masaaki, Takatsuki, Mitsuhisa, Kuroki, Tamotsu, Eguchi, Susumu |
| بيانات النشر: |
Elsevier BV |
| سنة النشر: |
2015 |
| المجموعة: |
NAOSITE: Nagasaki University Academic Output SITE / 長崎大学 学術研究成果リポジトリ |
| مصطلحات موضوعية: |
Cell sheet, Fibroblast, Hepatocyte transplantation, Human primary hepatocyte, Tissue engineering, Vascularization |
| الوصف: |
Subcutaneous liver tissue engineering is an attractive and minimally invasive approach used to curative treat hepatic failure and inherited liver diseases. However, graft failure occurs frequently due to insufficient infiltration of blood vessels (neoangiogenesis), while the maintenance of hepatocyte phenotype and function requires invivo development of the complex cellular organization of the hepatic lobule. Here we describe a subcutaneous human liver construction allowing for rapidly vascularized grafts by transplanting engineered cellular sheets consisting of human primary hepatocytes adhered onto a fibroblast layer. The engineered hepatocyte/fibroblast sheets (EHFSs) showed superior expression levels of vascularization-associated growth factors (vascular endothelial growth factor, transforming growth factor beta 1, and hepatocyte growth factor) invitro. EHFSs developed into vascularized subcutaneous human liver tissues contained glycogen stores, synthesized coagulation factor IX, and showed significantly higher synthesis rates of liver-specific proteins (albumin and alpha 1 anti-trypsin) invivo than tissues from hepatocyte-only sheets. The present study describes a new approach for vascularized human liver organogenesis under mouse skin. This approach could prove valuable for establishing novel cell therapies for liver diseases. ; Biomaterials, 65, pp.66-75; 2015 ; journal article |
| نوع الوثيقة: |
other/unknown material |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
01429612 |
| Relation: |
Biomaterials; 65; 66; 75; https://nagasaki-u.repo.nii.ac.jp/record/4860/files/Biomaterials65_66.pdf |
| الاتاحة: |
https://nagasaki-u.repo.nii.ac.jp/record/4860/files/Biomaterials65_66.pdf |
| Rights: |
c 2015 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.65416BE1 |
| قاعدة البيانات: |
BASE |