Academic Journal
Isolation, Extraction, and Characterization of Verotoxin-producing Escherichia coli O157:H7 from Diarrheal Stool Samples
| العنوان: | Isolation, Extraction, and Characterization of Verotoxin-producing Escherichia coli O157:H7 from Diarrheal Stool Samples |
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| المؤلفون: | Musthafa Poyil, Muhammad, Karuppiah, , Ponmurugan, S. S. Raja, Suresh, Sasikumar, P. |
| المصدر: | المجلة العالمية للعلوم الطبيه والصحية; مجلد 17 عدد 1 (2022): Volume 17 issue No 1; 116-127 ; Sudan Journal of Medical Sciences; Vol. 17 No. 1 (2022): Volume 17 issue No 1; 116-127 ; 1858-5051 ; 1858-8530 ; 10.52981/sjms.v17i1 |
| بيانات النشر: | Omdurman Islamic University. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | E. coli, Verotoxin, diarrhea, hemorrhagic colitis |
| الوصف: | Background: Escherichia coli O157:H7 infection causes hemorrhagic colitis andis diagnosed based on symptoms such as cramps, stomach pain, and waterydiarrhea. Shiga-like toxins (Verotoxin) produced by Escherichia coli O157:H7 damagesendothelial cells of both kidney and brain, causing renal dysfunction and neurologicalproblems.Methods: The present study focuses on identifying the prevalence of Verotoxinproducing Escherichia coli O157:H7 among diarrheal inpatients at Erode GovernmentHospital, India, and its antibiogram. Further, the Verotoxins were characterized byusing SDS-PAGE analysis. A total of 123 diarrheal stool samples were collected, and37 of them (30.08% of the total samples) were found to have the presence of E. coli.The organisms were identified based on their colony morphology on various media,cell morphology, and biochemical tests. The Shiga-like toxin production was identifiedby non-fermentation of sorbitol on SMAC agar plates. Confirmation of Shiga-like toxinwas performed using agglutination assay.Results: In total, 12 isolates showed agglutination and these isolates were confirmedto be E. coli O157:H7. The molecular weight of the Verotoxin was found to be between20 and 29 kD. The antibiogram profile of the four isolated strains against 10 standardantibiotics was determined.Conclusion: The results of this study show the occurrence of drug resistance onhemorrhagic colitis causing E. coli O157:H7 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://journal.oiu.edu.sd/index.php/FMHS/article/view/2602/1890; http://journal.oiu.edu.sd/index.php/FMHS/article/view/2602 |
| DOI: | 10.52981/sjms.v17i1.2602 |
| الاتاحة: | http://journal.oiu.edu.sd/index.php/FMHS/article/view/2602 https://doi.org/10.52981/sjms.v17i1.2602 |
| Rights: | Copyright (c) 2022 Sudan Journal of Medical Sciences |
| رقم الانضمام: | edsbas.65371C9F |
| قاعدة البيانات: | BASE |
| DOI: | 10.52981/sjms.v17i1.2602 |
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