Academic Journal
A simple and robust serum-free media for the proliferation of muscle cells
| العنوان: | A simple and robust serum-free media for the proliferation of muscle cells |
|---|---|
| المؤلفون: | Skrivergaard, Stig, Young, Jette Feveile, Sahebekhtiari, Navid, Semper, Cameron, Venkatesan, Meenakshi, Savchenko, Alexei, Stogios, Peter J., Therkildsen, Margrethe, Rasmussen, Martin Krøyer |
| المصدر: | Skrivergaard , S , Young , J F , Sahebekhtiari , N , Semper , C , Venkatesan , M , Savchenko , A , Stogios , P J , Therkildsen , M & Rasmussen , M K 2023 , ' A simple and robust serum-free media for the proliferation of muscle cells ' , Food Research International , vol. 172 , 113194 . https://doi.org/10.1016/j.foodres.2023.113194 |
| سنة النشر: | 2023 |
| المجموعة: | Aarhus University: Research |
| مصطلحات موضوعية: | Bovine satellite cells, Cultivated meat, Image-based cytometry, Serum-free media optimization, Transcriptomics |
| الوصف: | Cultivated meat production requires an efficient, robust and highly optimized serum-free cell culture media for the needed upscaling of muscle cell expansion. Existing formulations of serum-free media are complex, expensive and have not been optimized for muscle cells. Thus, we undertook this work to develop a simple and robust serum-free media for the proliferation of bovine satellite cells (SCs) through Design of Experiment (DOE) and Response Surface Methodology (RSM) using precise and high-throughput image-based cytometry. Proliferative attributes were investigated with transcriptomics and long-term performance was validated using multiple live assays. Here we formulated a media based on three highly optimized components; FGF2 (2 ng/mL), fetuin (600 µg/mL) and BSA (75 µg/mL) which together with an insulin-transferrin-selenium (1x) supplement, sustained the proliferation of bovine SCs, porcine SCs and murine C2C12 muscle cells. Remarkably, cells cultured in our media named Tri-basal 2.0+ performed better than cell cultured in 10% FBS, with respect to proliferation. Hence, the optimized Tri-basal 2.0+ enhanced serum-free cell attachment and long-term proliferation, providing an alternative solution to the use of FBS in the production of cultivated meat. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1016/j.foodres.2023.113194 |
| الاتاحة: | https://pure.au.dk/portal/en/publications/bc6d65dc-67de-48de-bd8b-d2e2070757d6 https://doi.org/10.1016/j.foodres.2023.113194 https://pure.au.dk/ws/files/358532191/1-s2.0-S0963996923007391-main.pdf http://www.scopus.com/inward/record.url?scp=85164222845&partnerID=8YFLogxK |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.65297EB7 |
| قاعدة البيانات: | BASE |
| ResultId |
1 |
|---|---|
| Header |
edsbas BASE edsbas.65297EB7 961 3 Academic Journal academicJournal 960.976379394531 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.65297EB7&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => https://pure.au.dk/portal/en/publications/bc6d65dc-67de-48de-bd8b-d2e2070757d6# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => A simple and robust serum-free media for the proliferation of muscle cells
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Skrivergaard%2C+Stig%22">Skrivergaard, Stig</searchLink><br /><searchLink fieldCode="AR" term="%22Young%2C+Jette+Feveile%22">Young, Jette Feveile</searchLink><br /><searchLink fieldCode="AR" term="%22Sahebekhtiari%2C+Navid%22">Sahebekhtiari, Navid</searchLink><br /><searchLink fieldCode="AR" term="%22Semper%2C+Cameron%22">Semper, Cameron</searchLink><br /><searchLink fieldCode="AR" term="%22Venkatesan%2C+Meenakshi%22">Venkatesan, Meenakshi</searchLink><br /><searchLink fieldCode="AR" term="%22Savchenko%2C+Alexei%22">Savchenko, Alexei</searchLink><br /><searchLink fieldCode="AR" term="%22Stogios%2C+Peter+J%2E%22">Stogios, Peter J.</searchLink><br /><searchLink fieldCode="AR" term="%22Therkildsen%2C+Margrethe%22">Therkildsen, Margrethe</searchLink><br /><searchLink fieldCode="AR" term="%22Rasmussen%2C+Martin+Krøyer%22">Rasmussen, Martin Krøyer</searchLink> ) Array ( [Name] => TitleSource [Label] => Source [Group] => Src [Data] => Skrivergaard , S , Young , J F , Sahebekhtiari , N , Semper , C , Venkatesan , M , Savchenko , A , Stogios , P J , Therkildsen , M & Rasmussen , M K 2023 , ' A simple and robust serum-free media for the proliferation of muscle cells ' , Food Research International , vol. 172 , 113194 . https://doi.org/10.1016/j.foodres.2023.113194 ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2023 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => Aarhus University: Research ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Bovine+satellite+cells%22">Bovine satellite cells</searchLink><br /><searchLink fieldCode="DE" term="%22Cultivated+meat%22">Cultivated meat</searchLink><br /><searchLink fieldCode="DE" term="%22Image-based+cytometry%22">Image-based cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22Serum-free+media+optimization%22">Serum-free media optimization</searchLink><br /><searchLink fieldCode="DE" term="%22Transcriptomics%22">Transcriptomics</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => Cultivated meat production requires an efficient, robust and highly optimized serum-free cell culture media for the needed upscaling of muscle cell expansion. Existing formulations of serum-free media are complex, expensive and have not been optimized for muscle cells. Thus, we undertook this work to develop a simple and robust serum-free media for the proliferation of bovine satellite cells (SCs) through Design of Experiment (DOE) and Response Surface Methodology (RSM) using precise and high-throughput image-based cytometry. Proliferative attributes were investigated with transcriptomics and long-term performance was validated using multiple live assays. Here we formulated a media based on three highly optimized components; FGF2 (2 ng/mL), fetuin (600 µg/mL) and BSA (75 µg/mL) which together with an insulin-transferrin-selenium (1x) supplement, sustained the proliferation of bovine SCs, porcine SCs and murine C2C12 muscle cells. Remarkably, cells cultured in our media named Tri-basal 2.0+ performed better than cell cultured in 10% FBS, with respect to proliferation. Hence, the optimized Tri-basal 2.0+ enhanced serum-free cell attachment and long-term proliferation, providing an alternative solution to the use of FBS in the production of cultivated meat. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article in journal/newspaper ) Array ( [Name] => Format [Label] => File Description [Group] => SrcInfo [Data] => application/pdf ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1016/j.foodres.2023.113194 ) Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => https://pure.au.dk/portal/en/publications/bc6d65dc-67de-48de-bd8b-d2e2070757d6<br />https://doi.org/10.1016/j.foodres.2023.113194<br />https://pure.au.dk/ws/files/358532191/1-s2.0-S0963996923007391-main.pdf<br />http://www.scopus.com/inward/record.url?scp=85164222845&partnerID=8YFLogxK ) Array ( [Name] => Copyright [Label] => Rights [Group] => Cpyrght [Data] => info:eu-repo/semantics/openAccess ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.65297EB7 ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1016/j.foodres.2023.113194
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Bovine satellite cells
[Type] => general
)
[1] => Array
(
[SubjectFull] => Cultivated meat
[Type] => general
)
[2] => Array
(
[SubjectFull] => Image-based cytometry
[Type] => general
)
[3] => Array
(
[SubjectFull] => Serum-free media optimization
[Type] => general
)
[4] => Array
(
[SubjectFull] => Transcriptomics
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => A simple and robust serum-free media for the proliferation of muscle cells
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Skrivergaard, Stig
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Young, Jette Feveile
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Sahebekhtiari, Navid
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Semper, Cameron
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Venkatesan, Meenakshi
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Savchenko, Alexei
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Stogios, Peter J.
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Therkildsen, Margrethe
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Rasmussen, Martin Krøyer
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 01
[Type] => published
[Y] => 2023
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-locals
[Value] => edsbas
)
[1] => Array
(
[Type] => issn-locals
[Value] => edsbas.oa
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => $2
[Type] => main
)
)
)
)
)
)
)
|
| IllustrationInfo |