Academic Journal
A simple and robust serum-free media for the proliferation of muscle cells
| العنوان: | A simple and robust serum-free media for the proliferation of muscle cells |
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| المؤلفون: | Skrivergaard, Stig, Young, Jette Feveile, Sahebekhtiari, Navid, Semper, Cameron, Venkatesan, Meenakshi, Savchenko, Alexei, Stogios, Peter J., Therkildsen, Margrethe, Rasmussen, Martin Krøyer |
| المصدر: | Skrivergaard , S , Young , J F , Sahebekhtiari , N , Semper , C , Venkatesan , M , Savchenko , A , Stogios , P J , Therkildsen , M & Rasmussen , M K 2023 , ' A simple and robust serum-free media for the proliferation of muscle cells ' , Food Research International , vol. 172 , 113194 . https://doi.org/10.1016/j.foodres.2023.113194 |
| سنة النشر: | 2023 |
| المجموعة: | Aarhus University: Research |
| مصطلحات موضوعية: | Bovine satellite cells, Cultivated meat, Image-based cytometry, Serum-free media optimization, Transcriptomics |
| الوصف: | Cultivated meat production requires an efficient, robust and highly optimized serum-free cell culture media for the needed upscaling of muscle cell expansion. Existing formulations of serum-free media are complex, expensive and have not been optimized for muscle cells. Thus, we undertook this work to develop a simple and robust serum-free media for the proliferation of bovine satellite cells (SCs) through Design of Experiment (DOE) and Response Surface Methodology (RSM) using precise and high-throughput image-based cytometry. Proliferative attributes were investigated with transcriptomics and long-term performance was validated using multiple live assays. Here we formulated a media based on three highly optimized components; FGF2 (2 ng/mL), fetuin (600 µg/mL) and BSA (75 µg/mL) which together with an insulin-transferrin-selenium (1x) supplement, sustained the proliferation of bovine SCs, porcine SCs and murine C2C12 muscle cells. Remarkably, cells cultured in our media named Tri-basal 2.0+ performed better than cell cultured in 10% FBS, with respect to proliferation. Hence, the optimized Tri-basal 2.0+ enhanced serum-free cell attachment and long-term proliferation, providing an alternative solution to the use of FBS in the production of cultivated meat. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1016/j.foodres.2023.113194 |
| الاتاحة: | https://pure.au.dk/portal/en/publications/bc6d65dc-67de-48de-bd8b-d2e2070757d6 https://doi.org/10.1016/j.foodres.2023.113194 https://pure.au.dk/ws/files/358532191/1-s2.0-S0963996923007391-main.pdf http://www.scopus.com/inward/record.url?scp=85164222845&partnerID=8YFLogxK |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.65297EB7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.foodres.2023.113194 |
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