Academic Journal
Reconstructing the Human Renal Vascular–Tubular Unit In Vitro
| العنوان: | Reconstructing the Human Renal Vascular–Tubular Unit In Vitro |
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| المؤلفون: | Rayner, Samuel G., Phong, Kiet T., Xue, Jun, Lih, Daniel, Shankland, Stuart J., Kelly, Edward J., Himmelfarb, Jonathan, Zheng, Ying |
| المساهمون: | National Institutes of Health |
| المصدر: | Advanced Healthcare Materials ; volume 7, issue 23 ; ISSN 2192-2640 2192-2659 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2018 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Engineered human kidney‐on‐a‐chip platforms show tremendous promise for disease modeling and drug screening. Outstanding challenges exist, however, in reconstructing the complex architecture, cellular make‐up, and matrix composition necessary for the proper modeling of kidney function. Herein, the first fully tunable human kidney‐on‐a‐chip platform is reported that allows the reconstruction of the native architecture of the renal endothelial–epithelial exchange interface using entirely cell‐remodelable matrix and patient‐derived kidney cells. This platform consists of a double‐layer human renal vascular–tubular unit (hRVTU) enabled by a thin collagen membrane that replicates the kidney exchange interface. It is shown that endothelial and epithelial cells lining their respective lumens remodel the membrane in culture into a ≈1 µm thick exchange interface composed of native basement membrane proteins. This interface displays sufficient mechanical integrity for media flow and blood perfusion. As a proof of principle, it is demonstrated that the hRVTU performs kidney‐specific functions including reabsorption of albumin and glucose from the epithelial channel. By incorporating multiple cell populations from single donors, it is demonstrated that the hRVTU may have utility for future precision medicine applications. The success of the system provides new opportunities for the next generation of organ‐on‐a‐chip models. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/adhm.201801120 |
| الاتاحة: | http://dx.doi.org/10.1002/adhm.201801120 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fadhm.201801120 https://onlinelibrary.wiley.com/doi/pdf/10.1002/adhm.201801120 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/adhm.201801120 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.64B3DA0C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/adhm.201801120 |
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