Academic Journal
Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia
| العنوان: | Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia |
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| المؤلفون: | Mandić, Marija R., Oalđe, Mariana M., Lunić, Tanja M., Sabovljević, Aneta D., Sabovljević, Marko S., Gašić, Uroš, Duletić-Laušević, Sonja N., Božić, Bojan Đ., Božić Nedeljković, Biljana Dj., Chen, Jen-Tsung |
| المصدر: | PLOS ONE |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2021 |
| المجموعة: | RADaR - Digital Repository of Archived Publications Institute for Biological Research "Sinisa Stankovic" / РАДаР - Репозиторијум Архивираних Дигиталних Радова Институт за биолошка истраживања "Синиша Станковић" |
| مصطلحات موضوعية: | Antioxidants, Apoptosis, Ethanol, Mosses, Phenols, Serine proteases, Terpenes, Vitamin C |
| الوصف: | Bioactive compounds from natural sources are of great importance because of their potential pharmacological activity and tremendous structural diversity. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. have been examined, as well as their antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative potential. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical characterization of the extracts revealed the presence of phenolics and flavonoid compounds, together with triterpenoids as secondary metabolites of high biological activity. Significant antioxidant properties of all the extracts were exhibited using the β-carotene assay. The highest activities were found for water:ethanol extract (with the highest inhibition rate of 96%), but also significant inhibition was measured for ethanol and ethyl acetate extracts (80% and 70%, respectively). Confirmation of biocompatibility of investigated moss extracts has been performed using normal human fibroblast cell line, MRC-5. The H . ciliata extracts exhibited significant antiproliferative activity (~ 50%) against the MDA-MB-231 (human breast adenocarcinoma cell line), which has not previously been reported elsewhere. The Griess assay confirmed the potential anti-neuroinflammatory activity of the extracts, as significant effects in reducing NO production by LPS-stimulated BV2 (normal murine microglia cell line) was observed. This data is in line with noted antineurodegenerative potential measured by the inhibition of acetylcholinesterase (with the highest inhibition rate of 60% for ethyl acetate extract) and tyrosinase (with the highest inhibition rate of 70% for ethanol extract). Additionally, the H . ciliata extracts exhibited significant antidiabetic effect mediated by α-glucosidase inhibition (with the highest inhibition rate of 80% for ethyl acetate extract). The obtained data suggest the presence of immunomodulatory effects of the moss ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1932-6203 |
| Relation: | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS//; info:eu-repo/grantAgreement/MESTD/inst-2020/200178/RS//; internal-pdf://Mandić et al. - 2021 - Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (H.pdf; https://dx.plos.org/10.1371/journal.pone.0246810; https://radar.ibiss.bg.ac.rs/handle/123456789/4156; 2-s2.0-85101409951; 000618274000001; https://radar.ibiss.bg.ac.rs/bitstream/id/8262/journal.pone.0246810.pdf |
| DOI: | 10.1371/journal.pone.0246810 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0246810 https://radar.ibiss.bg.ac.rs/handle/123456789/4156 https://radar.ibiss.bg.ac.rs/bitstream/id/8262/journal.pone.0246810.pdf |
| Rights: | openAccess ; https://creativecommons.org/licenses/by/4.0/ ; BY ; © 2021 Mandić et al. |
| رقم الانضمام: | edsbas.64B08551 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0246810 |