Academic Journal
Dissociation of Akt1 from its negative regulator JIP1 is mediated through the ASK1�밠EK�밓NK signal transduction pathway during metabolic oxidative stress: A negative feedback loop
| العنوان: | Dissociation of Akt1 from its negative regulator JIP1 is mediated through the ASK1�밠EK�밓NK signal transduction pathway during metabolic oxidative stress: A negative feedback loop |
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| المساهمون: | Jae J. Song, Yong J. Lee, Song, Jae Jin |
| المصدر: | T200504611.pdf |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Adaptor Proteins, Signal Transducing/genetics, Signal Transducing/metabolism, Cell Line, Tumor, Down-Regulation, Enzyme Activation, Feedback, Physiological/physiology, Glucose/deficiency, Humans, MAP Kinase Kinase 1/metabolism, MAP Kinase Kinase 4/metabolism, MAP Kinase Kinase Kinase 5/metabolism, MAP Kinase Signaling System/physiology, Mitogen-Activated Protein Kinase 8/metabolism, Mitogen-Activated Protein Kinase 9/metabolism, Oxidative Stress/physiology, Phosphorylation, Point Mutation/genetics, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, Proto-Oncogene Proteins/genetics, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-akt, RNA Interference/physiology, Signal Transduction/physiology |
| Time: | 15998799 |
| الوصف: | We have previously observed that metabolic oxidative stress�밿nduced death domain�밶ssociated protein (Daxx) trafficking is mediated by the ASK1�밪EK1�밓NK1�밐IPK1 signal transduction pathway. The relocalized Daxx from the nucleus to the cytoplasm during glucose deprivation participates in a positive regulatory feedback loop by binding to apoptosis signal�뱑egulating kinase (ASK) 1. In this study, we report that Akt1 is involved in a negative regulatory feedback loop during glucose deprivation. Akt1 interacts with c-Jun NH2-terminal kinase (JNK)�밿nteracting protein (JIP) 1, and Akt1 catalytic activity is inhibited. The JNK2-mediated phosphorylation of JIP1 results in the dissociation of Akt1 from JIP1 and subsequently restores Akt1 enzyme activity. Concomitantly, Akt1 interacts with stress-activated protein kinase/extracellular signal�뱑egulated kinase (SEK) 1 (also known as MKK4) and inhibits SEK1 activity. Knockdown of SEK1 leads to the inhibition of JNK activation, JIP1�밓NK2 binding, and the dissociation of Akt1 from JIP1 during glucose deprivation. Knockdown of JIP1 also leads to the inhibition of JNK activation, whereas the knockdown of Akt1 promotes JNK activation during glucose deprivation. Altogether, our data demonstrate that Akt1 participates in a negative regulatory feedback loop by interacting with the JIP1 scaffold protein. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 61~72 |
| اللغة: | unknown |
| تدمد: | 0021-9525 1540-8140 |
| Relation: | JOURNAL OF CELL BIOLOGY; J01300; OAK-2005-02220; https://ir.ymlib.yonsei.ac.kr/handle/22282913/114961; T200504611; JOURNAL OF CELL BIOLOGY, Vol.170(1) : 61-72, 2005 |
| DOI: | 10.1083/jcb.200502070 |
| الاتاحة: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114961 https://doi.org/10.1083/jcb.200502070 |
| Rights: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ ; free |
| رقم الانضمام: | edsbas.641B3F50 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00219525 15408140 |
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| DOI: | 10.1083/jcb.200502070 |