Academic Journal
Effect of pirfenidone injection on ferret vocal fold scars: A preliminary in vivo study
| العنوان: | Effect of pirfenidone injection on ferret vocal fold scars: A preliminary in vivo study |
|---|---|
| المؤلفون: | Yamada, Takao, Kumai, Yoshihiko, Kodama, Haruka, Nishimoto, Kohei, Miyamaru, Satoru, Onoue, Satomi, Orita, Yorihisa |
| المصدر: | The Laryngoscope ; volume 130, issue 3, page 726-731 ; ISSN 0023-852X 1531-4995 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2019 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Objectives This study examined the antifibrotic effect of pirfenidone (PFD), which has received regulatory approval in the United States and Japan for treatment of idiopathic pulmonary fibrosis, on the scarred ferret vocal fold (VF) in vivo. Methods Eight male ferrets were divided into two groups: saline and PFD. All animals underwent unilateral scarring under anesthesia. The right VF was electrocauterized with ablation of the entire lamina propria. PFD (1.0 mg/mL) or saline injections into right‐side scarred VFs were performed (under an operating microscope) 4 weeks later. After an additional 4 weeks, the larynges were harvested for histological analysis. Prior to harvesting, the ferrets were re‐anesthetized, and the VFs were observed and recorded using a rigid video laryngoscope. We immunohistochemically evaluated the expression of collagen types I and III, alpha‐smooth muscle actin (α‐SMA), and fibronectin in the entire lamina propria. We compared the affected areas (calculated using ImageJ software) between the treated (right) and untreated (left) sides within the same animals and between groups. Results Collagen type I ( P = 0.0021) and α‐SMA ( P = 0.0021) expression levels were lower in the PFD group, but the collagen type III and fibronectin levels did not differ significantly between the two groups. Conclusion PFD injection into the scarred VF is a potentially promising novel antifibrotic treatment. Level of Evidence NA Laryngoscope , 130:726–731, 2020 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/lary.28087 |
| الاتاحة: | https://doi.org/10.1002/lary.28087 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lary.28087 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lary.28087 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.640E09 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/lary.28087 |
|---|