Academic Journal
Rapid On-Site Detection of Arboviruses by a Direct RT-qPCR Assay
| العنوان: | Rapid On-Site Detection of Arboviruses by a Direct RT-qPCR Assay |
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| المؤلفون: | Mhamadi, Moufid, Mencattelli, Giulia, Gaye, Alioune, Ndiaye, El, Hadji, Sow, Aïssatou, Aïcha, Faye, Martin, Ndione, Marie, Henriette Dior, Diagne, Moussa, Moïse, Mhamadi, Moundhir, Faye, Ousmane, Weidmann, Manfred, Faye, Oumar, Diallo, Mawlouth, Diagne, Cheikh, Tidiane |
| المساهمون: | Département de virologie - Virology Department Dakar, Institut Pasteur de Dakar, Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur), Pôle Zoologie médicale Dakar, Diatropix Dakar, Sénégal, Medizinische Hochschule Brandenburg Theodor Fontane / Brandenburg Medical School Theodor-Fontane (MHB Theodor Fontane), This project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking (JU) under grant agreement No 823666. The JU receives support from the European Union’s Horizon 2020 research and innovation program, and EFPIA and CEPHEID EUROPE SAS. |
| المصدر: | ISSN: 2079-6374 ; Biosensors ; https://hal.science/hal-04569994 ; Biosensors, 2023, 13 (12), pp.1035. ⟨10.3390/bios13121035⟩. |
| بيانات النشر: | CCSD MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| مصطلحات موضوعية: | direct RT-qPCR supernatant arbovirus infected mosquitoes field diagnosis, direct RT-qPCR, supernatant, arbovirus, infected mosquitoes, field diagnosis, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Arthropod-borne diseases currently constitute a source of major health concerns worldwide. They account for about 50% of global infectious diseases and cause nearly 700,000 deaths every year. Their rapid increase and spread constitute a huge challenge for public health, highlighting the need for early detection during epidemics, to curtail the virus spread, and to enhance outbreak management. Here, we compared a standard quantitative polymerase chain reaction (RT-qPCR) and a direct RT-qPCR assay for the detection of Zika (ZIKV), Chikungunya (CHIKV), and Rift Valley Fever (RVFV) viruses from experimentally infected-mosquitoes. The direct RT-qPCR could be completed within 1.5 h and required 1 μL of viral supernatant from homogenized mosquito body pools. Results showed that the direct RT-qPCR can detect 85.71%, 89%, and 100% of CHIKV, RVFV, and ZIKV samples by direct amplifications compared to the standard method. The use of 1:10 diluted supernatant is suggested for CHIKV and RVFV direct RT-qPCR. Despite a slight drop in sensitivity for direct PCR, our technique is more affordable, less time-consuming, and provides a better option for qualitative field diagnosis during outbreak management. It represents an alternative when extraction and purification steps are not possible because of insufficient sample volume or biosecurity issues. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38131795; PUBMED: 38131795; PUBMEDCENTRAL: PMC10741549 |
| DOI: | 10.3390/bios13121035 |
| الاتاحة: | https://hal.science/hal-04569994 https://hal.science/hal-04569994v1/document https://hal.science/hal-04569994v1/file/Mhamadi%20et%20al.%20-%202023%20-%20Rapid%20On-Site%20Detection%20of%20Arboviruses%20by%20a%20Direct.pdf https://doi.org/10.3390/bios13121035 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.63CFFA28 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/bios13121035 |
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