Academic Journal
Identification of two human nuclear proteins that recognise the cytosine-rich strand of human telomeres in vitro
| العنوان: | Identification of two human nuclear proteins that recognise the cytosine-rich strand of human telomeres in vitro |
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| المؤلفون: | Lacroix, L., Lienard, H., Labourier, E., Djavaheri-Mergny, M., Lacoste, J., Leffers, H., Tazi, J., Helene, C., Mergny, J. L. |
| المساهمون: | Régulation et dynamique des génomes, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines Lyon (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0305-1048. |
| بيانات النشر: | HAL CCSD Oxford University Press |
| سنة النشر: | 2000 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | DNA-binding protein sf2/asf splicing factor g-quartet formation saccharomyces-cerevisiae in-vitro yeast telomeres c-myc ribonucleoprotein-k circular-dichroism trypanosoma-brucei, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | Most studies on the structure of DNA in telomeres have been dedicated to the double-stranded region or the guanosine-rich strand and consequently little is known about the factors that may bind to the telomere cytosine-rich (C-rich) strand. This led us to investigate whether proteins exist that can recognise C-rich sequences. We have isolated several nuclear factors from human cell extracts that specifically bind the C-rich strand of vertebrate telomeres [namely a d(CCCTAA)(n) repeat] with high affinity and bind double-stranded telomeric DNA with a 100x reduced affinity. A biochemical assay allowed us to characterise four proteins of apparent molecular weights 66-64, 45 and 35 kDa, respectively. To identify these polypeptides we screened a lambda gt11-based cDNA expression library, obtained from human HeLa cells using a radiolabelled telomeric oligonucleotide as a probe. Two clones were purified and sequenced: the first corresponded to the hnRNP K protein and the second to the ASF/SF2 splicing factor. Confirmation of the screening results was obtained with recombinant proteins, both of which bind to the human telomeric C-rich strand in vitro. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-02196848; https://hal.science/hal-02196848; PUBMEDCENTRAL: PMC102786 |
| DOI: | 10.1093/nar/28.7.1564 |
| الاتاحة: | https://hal.science/hal-02196848 https://doi.org/10.1093/nar/28.7.1564 |
| رقم الانضمام: | edsbas.63569B67 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/28.7.1564 |
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