Academic Journal
经皮反复给予玉红膏对大鼠器官毒性的研究 ; Organ toxicity of Yuhong Ointment(玉红膏) after repeated transdermal administration in rats
| العنوان: | 经皮反复给予玉红膏对大鼠器官毒性的研究 ; Organ toxicity of Yuhong Ointment(玉红膏) after repeated transdermal administration in rats |
|---|---|
| المؤلفون: | 胡小靖, 孙新民, 黄雯, 邱恒, 牟稷征, 王丽霞, 王旗 |
| المساهمون: | 100191,北京大学公共卫生学院毒理学系, 中国中医科学院广安门医院药剂科 |
| المصدر: | 万方 ; 知网 ; http://d.g.wanfangdata.com.cn/Periodical_ywblfyzz201305004.aspx |
| بيانات النشر: | 药物不良反应杂志 |
| سنة النشر: | 2013 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | 玉红膏, 经皮给药, 大鼠, 器官毒性, Yuhong Ointment, Transdermal administration, Rat, Organ toxicity |
| الوصف: | 目的 观察经皮反复给予玉红膏对大鼠肝、肾功能及心、脑、肝、肾、脾等主要脏器组织形态学的影响,为临床安全用药提供实验数据.方法 SPF级SD大鼠100只,体重200 g,雌雄各半.用抽签法随机分为基质对照组、1倍浓度玉红膏组(轻粉含量0.4％)、2倍浓度玉红膏组(轻粉含量0.8％)、4倍浓度玉红膏组(轻粉含量1.6％)和1.6％轻粉组,每组20只.制备大鼠皮肤破损模型,经破损皮肤涂抹不同浓度玉红膏,1次/d,连续28 d.分别于给药前、给药14、28 d和停药28 d测定各组大鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、尿素氮(BUN)、肌酐(Cr)和N-乙酰-β-D氨基葡萄糖苷酶(NAG)水平;给药28 d和停药28 d时每组各处死10只大鼠,取心、脑、肝、肾、脾等脏器称重,计算脏器系数,并取肝、肾、脾组织观察形态学改变.结果 不同浓度玉红膏组和1.6％轻粉组大鼠ALT、AST、BUN、Cr和NAG水平与同期基质对照组比较差异无统计学意义(均P＞0.05);停药28 d时,1.6％轻粉组大鼠血清Cr含量高于基质对照组(P＜0.05).给药28 d与停药28d时,2和4倍浓度玉红膏组、1.6％轻粉组大鼠的肾脏系数均明显高于基质对照组(均P＜0.05).各组大鼠心、脑、肝、脾脏器系数的差异均无统计学意义(均P＞0.05).给药28 d时各组大鼠肝、肾、脾组织均无明显形态学改变.结论 经皮反复给予玉红膏未发现大鼠出现明显的器官毒性反应,高浓度的玉红膏可能导致大鼠发生可逆转的肾损伤.提示临床长期大量应用玉红膏应监测患者的肾功能. ; Objective To observe the effects of Yuhong Ointment on rat's liver and kidney functions and histomorphology of heart,brain,liver,kidney and spleen after transdermal administration repeatedly,in order to provide experimental evidences for safe use of Yuhong Ointment in clinical practice.Methods A total of 100 SPF SD rats of equal number of both genders,weighting 200 g,were divided into 5 groups:matrix control group,1 time concentration of Yuhong Ointment group (containing 0.4％ calomel),2 times concentration of Yuhong Ointment group (containing 0.8％ calomel),4 times concentration of Yuhong Ointment group (containing 1.6％ calomel) and calomel group (containing 1.6％ calomel) by drawn lots randomly,each group comprised 20 rats.The model of rat's skin injury was prepared.Yuhong Ointment in different concentrations were applied on the skin-impaired once daily for 28 days.The levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),blood urea nitrogen (BUN),creatinine (Cr) and N-acetyl beta-D glucosaminidase (NAG) of rats in different groups were measured before treatment,14 and 28 days after treatment,and 28 days after drug withdrawal,respectively.Ten rats in every group were sacrificed on 28 days after treatment and 28 days after drug withdrawal,respectively.The heart,brain,liver,kidney,and spleen of rats in different groups were ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | Chinese |
| تدمد: | 1008-5734 |
| Relation: | 药物不良反应杂志.2013,15,(5),248-253.; 890965; http://hdl.handle.net/20.500.11897/30263 |
| DOI: | 10.3760/cma.j.issn.1008-5734.2013.05.004 |
| الاتاحة: | https://hdl.handle.net/20.500.11897/30263 https://doi.org/10.3760/cma.j.issn.1008-5734.2013.05.004 |
| رقم الانضمام: | edsbas.632F4395 |
| قاعدة البيانات: | BASE |
| تدمد: | 10085734 |
|---|---|
| DOI: | 10.3760/cma.j.issn.1008-5734.2013.05.004 |