Academic Journal
Contribution of SARS-CoV-2 infection preceding COVID-19 mRNA vaccination to generation of cellular and humoral immune responses in children
| العنوان: | Contribution of SARS-CoV-2 infection preceding COVID-19 mRNA vaccination to generation of cellular and humoral immune responses in children |
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| المؤلفون: | Verheul, Marije K., Vos, Martijn, de Rond, Lia, De Zeeuw-Brouwer, Mary-Lène, Nijhof, Kim H., Smit, Debbie, Oomen, Debbie, Molenaar, Petra, Bogaard, Marjan, van Bergen, Rianne, Middelhof, Irene, Beckers, Lisa, Wijmenga-Monsuur, Alienke J., Buisman, Anne-Marie, Boer, Mardi C., van Binnendijk, Rob, de Wit, Jelle, Guichelaar, Teun |
| المصدر: | Frontiers in Immunology ; volume 14 ; ISSN 1664-3224 |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2023 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | Primary COVID-19 vaccination for children, 5-17 years of age, was offered in the Netherlands at a time when a substantial part of this population had already experienced a SARS-CoV-2 infection. While vaccination has been shown effective, underlying immune responses have not been extensively studied. We studied immune responsiveness to one and/or two doses of primary BNT162b2 mRNA vaccination and compared the humoral and cellular immune response in children with and without a preceding infection. Antibodies targeting the original SARS-CoV-2 Spike or Omicron Spike were measured by multiplex immunoassay. B-cell and T-cell responses were investigated using enzyme-linked immunosorbent spot (ELISpot) assays. The activation of CD4 + and CD8 + T cells was studied by flowcytometry. Primary vaccination induced both a humoral and cellular adaptive response in naive children. These responses were stronger in those with a history of infection prior to vaccination. A second vaccine dose did not further boost antibody levels in those who previously experienced an infection. Infection-induced responsiveness prior to vaccination was mainly detected in CD8 + T cells, while vaccine-induced T-cell responses were mostly by CD4 + T cells. Thus, SARS-CoV-2 infection prior to vaccination enhances adaptive cellular and humoral immune responses to primary COVID-19 vaccination in children. As most children are now expected to contract infection before the age of five, the impact of infection-induced immunity in children is of high relevance. Therefore, considering natural infection as a priming immunogen that enhances subsequent vaccine-responsiveness may help decision-making on the number and timing of vaccine doses. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fimmu.2023.1327875 |
| DOI: | 10.3389/fimmu.2023.1327875/full |
| الاتاحة: | http://dx.doi.org/10.3389/fimmu.2023.1327875 https://www.frontiersin.org/articles/10.3389/fimmu.2023.1327875/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.6324E303 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2023.1327875 |
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