Academic Journal
Oleic acid stimulation of amino acid uptake in primary human trophoblast cells is mediated by phosphatidic acid and mTOR signaling
| العنوان: | Oleic acid stimulation of amino acid uptake in primary human trophoblast cells is mediated by phosphatidic acid and mTOR signaling |
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| المؤلفون: | Silva, Elena, Ferchaud‐roucher, Véronique, Kramer, Anita, Madi, Lana, Pantham, Priyadarshini, Chassen, Stephanie, Jansson, Thomas, Powell, Theresa, L |
| المساهمون: | University of Colorado Anschutz Medical Campus Aurora, Physiopathologie des Adaptations Nutritionnelles (PhAN), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Univ Calif San Diego, Scripps Inst Oceanog, Geosci Res Div, Scripps Isotope Geochem Lab, La Jolla, CA 92093 USA |
| المصدر: | ISSN: 2573-9832 ; FASEB BioAdvances ; https://hal.inrae.fr/hal-04530570 ; FASEB BioAdvances, 2024, 6 (1), pp.1-11. ⟨10.1096/fba.2023-00113⟩. |
| بيانات النشر: | CCSD John Wiley & Sons, Inc., [2019]- |
| سنة النشر: | 2024 |
| المجموعة: | Université de Nantes: HAL-UNIV-NANTES |
| مصطلحات موضوعية: | fatty acids, human, Kennedy pathway, maternal-fetal exchange, mechanistic target of rapamycin, phosphatidic acid, placenta, pregnancy, acylglycerol-3-phosphate-O-acyltransferase DHA, docosahexaenoic acid GPAT, glycerol- primary human trophoblast PL, phospholipids siRNA, small interfering RNA maternal-fetal exchange, acylglycerol-3-phosphate-O-acyltransferase, DHA, docosahexaenoic acid, GPAT, glycerol- primary human trophoblast, PL, phospholipids, siRNA, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| الوصف: | International audience ; Abstract Normal fetal development is critically dependent on optimal nutrient supply by the placenta, and placental amino acid transport has been demonstrated to be positively associated with fetal growth. Mechanistic target of rapamycin (mTOR) is a positive regulator of placental amino acid transporters, such as System A. Oleic acid (OA) has been previously shown to have a stimulatory role on placental mTOR signaling and System A amino acid uptake in primary human trophoblast (PHT) cells. We investigated the mechanistic link between OA and System A activity in PHT. We found that inhibition of mTOR complex 1 or 2, using small interfering RNA to knock down raptor or rictor, prevented OA‐stimulated System A amino acid transport indicating the interaction of OA with mTOR. Phosphatidic acid (PA) is a key intermediary for phospholipid biosynthesis and a known regulator of the mTOR pathway; however, phospholipid biosynthetic pathways have not been extensively studied in placenta. We identified placental isoforms of acyl transferase enzymes involved in de novo phospholipid synthesis. Silencing of 1‐acylglycerol‐3‐phosphate‐O‐acyltransferase‐4, an enzyme in this pathway, prevented OA mediated stimulation of mTOR and System A amino acid transport. These data indicate that OA stimulates mTOR and amino acid transport in PHT cells mediated through de novo synthesis of PA. We speculate that fatty acids in the maternal circulation, such as OA, regulate placental functions critical for fetal growth by interaction with mTOR and that late pregnancy hyperlipidemia may be critical for increasing nutrient transfer to the fetus. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38223199; PUBMED: 38223199; WOS: 001100787500001 |
| DOI: | 10.1096/fba.2023-00113 |
| الاتاحة: | https://hal.inrae.fr/hal-04530570 https://hal.inrae.fr/hal-04530570v1/document https://hal.inrae.fr/hal-04530570v1/file/2023_Silva_Faseb_BioAdv.pdf https://doi.org/10.1096/fba.2023-00113 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.631093 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1096/fba.2023-00113 |
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