التفاصيل البيبلوغرافية
| العنوان: |
S1 Raw images - |
| المؤلفون: |
Mengting Yao, Chucun Fang, Zilong Wang, Tianting Guo, Dongwen Wu, Jiacheng Ma, Jian Wu, Jianwen Mo |
| سنة النشر: |
2024 |
| المجموعة: |
Smithsonian Institution: Figshare |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Cell Biology, Molecular Biology, Pharmacology, Biotechnology, Immunology, Marine Biology, Cancer, Infectious Diseases, Virology, Chemical Sciences not elsewhere classified, provides new ideas, potential molecular processes, nf -&# 954, like receptor 2, important pathogenic mechanisms, employed western blot, constructed lentiviral vectors, neutrophil extracellular traps, endothelial cell injury, determine whether mir, induced huvecs model, huvecs via inhibition, inhibiting neutrophil migration, used cellular tests, group &# 8217, glucose deprivation injury, b signaling pathway, +mir%22">xlink "> mir |
| الوصف: |
Background Endothelial cell injury is one of the important pathogenic mechanisms in thrombotic diseases, and also neutrophils are involved. MicroRNAs (miRNAs) have been demonstrated to act as essential players in endothelial cell injury, but the potential molecular processes are unknown. In this study, we used cellular tests to ascertain the protective effect of miR-328-3p on human umbilical vein endothelial cells (HUVECs) treated with oxygen-glucose deprivation (OGD). Methods In our study, an OGD-induced HUVECs model was established, and we constructed lentiviral vectors to establish stable HUVECs cell lines. miR-328-3p and Toll-like receptor 2 (TLR2) interacted, as demonstrated by the dual luciferase reporter assay. We used the CCK8, LDH release, and EdU assays to evaluate the proliferative capacity of each group of cells. To investigate the expression of TLR2, p-P65 NF-κB, P65 NF-κB, NLRP3, IL-1β, and IL-18, we employed Western blot and ELISA. Following OGD, each group’s cell supernatants were gathered and co-cultured with neutrophils. An immunofluorescence assay and Transwell assay have been performed to determine whether miR-328-3p/TLR2 interferes with neutrophil migration and neutrophil extracellular traps (NETs) formation. Results In OGD-treated HUVECs, the expression of miR-328-3p is downregulated. miR-328-3p directly targets TLR2, inhibits the NF-κB signaling pathway, and reverses the proliferative capacity of OGD-treated HUVECs, while inhibiting neutrophil migration and neutrophil extracellular trap formation. Conclusions miR-328-3p inhibits the NF-κB signaling pathway in OGD-treated HUVECs while inhibiting neutrophil migration and NETs formation, and ameliorating endothelial cell injury, which provides new ideas for the pathogenesis of thrombotic diseases. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/S1_Raw_images_-/25280021 |
| DOI: |
10.1371/journal.pone.0299382.s001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0299382.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.62C406A1 |
| قاعدة البيانات: |
BASE |