Academic Journal
Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides
| العنوان: | Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides |
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| المؤلفون: | Lv, Ling Ling, Li, Li Yun, Xiao, Long Qian, Pi, Jian Hui |
| المساهمون: | the Key Project of Hunan Provincial Department of Education |
| المصدر: | BMC Biotechnology ; volume 24, issue 1 ; ISSN 1472-6750 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2024 |
| الوصف: | Background Flavonoids are one of the bioactive ingredients of Lonicera macranthoides ( L. macranthoides ), however, their biosynthesis in the flower is still unclear. In this study, combined transcriptomic and targeted metabolomic analyses were performed to clarify the flavonoids biosynthesis during flowering of L. macranthoides . Results In the three sample groups, GB_vs_WB, GB_vs_WF and GB_vs_GF, there were 25, 22 and 18 differentially expressed genes (DEGs) in flavonoids biosynthetic pathway respectively. A total of 339 flavonoids were detected and quantified at four developmental stages of flower in L. macranthoides . In the three sample groups, 113, 155 and 163 differentially accumulated flavonoids (DAFs) were detected respectively. Among the DAFs, most apigenin derivatives in flavones and most kaempferol derivatives in flavonols were up-regulated. Correlation analysis between DEGs and DAFs showed that the down-regulated expressions of the CHS, DFR, C4H, F3’H , CCoAOMT_ 32 and the up-regulated expressions of the two HCT s resulted in down-regulated levels of dihydroquercetin, epigallocatechin and up-regulated level of kaempferol-3-O-(6’’-O-acetyl)-glucoside, cosmosiin and apigenin-4’-O-glucoside. The down-regulated expressions of F3H and FLS decreased the contents of 7 metabolites, including naringenin chalcone, proanthocyanidin B2, B3, B4, C1, limocitrin-3,7-di-O-glucoside and limocitrin-3-O-sophoroside. Conclusion The findings are helpful for genetic improvement of varieties in L.macranthoides . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s12896-024-00846-5 |
| DOI: | 10.1186/s12896-024-00846-5.pdf |
| DOI: | 10.1186/s12896-024-00846-5/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s12896-024-00846-5 https://link.springer.com/content/pdf/10.1186/s12896-024-00846-5.pdf https://link.springer.com/article/10.1186/s12896-024-00846-5/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.62B56368 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12896-024-00846-5 |
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