Academic Journal
Isopeptide bonding in planta allows functionalization of elongated flexuous proteinaceous viral nanoparticles, including non-viable constructs by other means
| العنوان: | Isopeptide bonding in planta allows functionalization of elongated flexuous proteinaceous viral nanoparticles, including non-viable constructs by other means |
|---|---|
| المؤلفون: | Truchado Martín, Daniel Alejandro, Rincón, Sara, Zurita, Lucía, Sánchez, Flora, Ponz, Fernando |
| المساهمون: | Guo, Huichen, Abdullah, SahibzadaWaheed |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Universidad Complutense de Madrid (UCM): E-Prints Complutense |
| مصطلحات موضوعية: | 577.2, Potyvirus, Functionalization, VLPs, Spytag/SpyCatcher, VIP, Biotechnology, Nanoparticle, Biología molecular (Biología), Bioquímica (Química), Biotecnología, Microbiología (Biología), 2302 Bioquímica, 2415.01 Biología Molecular de Microorganismos, 2420 Virología, 2414 Microbiología |
| Time: | 578, 579 |
| الوصف: | Plant viral nanoparticles (VNPs) have become an attractive platform for the development of novel nanotools in the last years because of their safety, inexpensive production, and straightforward functionalization. Turnip mosaic virus (TuMV) is one example of a plant-based VNP used as a nanobiotechnological platform either as virions or as virus-like particles (VLPs). Their functionalization mainly consists of coating their surface with the molecules of interest via chemical conjugation or genetic fusion. However, because of their limitations, these two methods sometimes result in non-viable constructs. In this paper, we applied the SpyTag/SpyCatcher technology as an alternative for the functionalization of TuMV VLPs with peptides and proteins. We chose as molecules of interest the green fluorescent protein (GFP) because of its good traceability, as well as the vasoactive intestinal peptide (VIP), given the previous unsuccessful attempts to functionalize TuMV VNPs by other methods. The successful conjugation of VLPs to GFP and VIP using SpyTag/SpyCatcher was confirmed through Western blot and electron microscopy. Moreover, the isopeptide bond between SpyTag and SpyCatcher occurred in vivo in co-agroinfiltrated Nicotiana benthamiana plants. These results demonstrated that SpyTag/SpyCatcher improves TuMV functionalization compared with previous approaches, thus implying the expansion of the application of the technology to elongated flexuous VNPs. ; Unión Europea ; Ministerio de Universidades (España) ; Comunidad de Madrid ; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) ; Universidad Complutense de Madrid (UCM) ; Depto. de Genética, Fisiología y Microbiología ; Fac. de Ciencias Biológicas ; TRUE ; pub |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| Relation: | ARIMNet-2 618127; Beca postdoctoral Margarita Salas; P2018/BAA-4574 , COV20/00114 , PanGreen-CM; RTA2015-00017-00-00; CT31/21; https://hdl.handle.net/20.500.14352/102837; Truchado, D. A., Rincón, S., Zurita, L., Sánchez, F., & Ponz, F. (2023). Isopeptide Bonding In Planta Allows Functionalization of Elongated Flexuous Proteinaceous Viral Nanoparticles, including Non-Viable Constructs by Other Means. Viruses, 15(2), 375.; https://www.mdpi.com/1999-4915/15/2/375 |
| DOI: | 10.3390/v15020375 |
| الاتاحة: | https://hdl.handle.net/20.500.14352/102837 https://doi.org/10.3390/v15020375 https://www.mdpi.com/1999-4915/15/2/375 |
| Rights: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; open access |
| رقم الانضمام: | edsbas.61885705 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19994915 |
|---|---|
| DOI: | 10.3390/v15020375 |