Academic Journal
In Situ Preparation of Tannic Acid-Modified Poly( N -isopropylacrylamide) Hydrogel Coatings for Boosting Cell Response
| العنوان: | In Situ Preparation of Tannic Acid-Modified Poly( N -isopropylacrylamide) Hydrogel Coatings for Boosting Cell Response |
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| المؤلفون: | Jufei Xu, Xiangzhe Liu, Pengpeng Liang, Hailong Yuan, Tianyou Yang |
| المصدر: | Pharmaceutics, Vol 16, Iss 4, p 538 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | modified poly( N -isopropylacrylamide) coatings, coating properties, protein behaviors, cell response, Pharmacy and materia medica, RS1-441 |
| الوصف: | The improvement of the capability of poly( N -isopropylacrylamide) (PNIPAAm) hydrogel coating in cell adhesion and detachment is critical to efficiently prepare cell sheets applied in cellular therapies and tissue engineering. To enhance cell response on the surface, the amine group-modified PNIPAAm (PNIPAAm-APTES) nanohydrogels were synthesized and deposited spontaneously on tannic acid (TA)-modified polyethylene (PE) plates. Subsequently, TA was introduced onto PNIPAAm-APTES nanohydrogels to fabricate coatings composed of TA-modified PNIPAAm-APTES (PNIPAAm-APTES-TA). Characterization techniques, including TEM, SEM, XPS, and UV-Vis spectroscopy, confirmed the effective deposition of hydrogels of PNIPAAm as well as the morphologies, content of chemical bonding-TA, and stability of various coatings. Importantly, the porous hydrogel coatings exhibited superhydrophilicity at 20 °C and thermo-responsive behavior. The fluorescence measurement demonstrated that the coating’s stability effectively regulated protein behavior, influencing cell response. Notably, cell response tests revealed that even without precise control over the chain length/thickness of PNIPAAm during synthesis, the coatings enhanced cell adhesion and detachment, facilitating efficient cell culture. This work represented a novel and facile approach to preparing bioactive PNIPAAm for cell culture. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1999-4923 |
| Relation: | https://www.mdpi.com/1999-4923/16/4/538; https://doaj.org/toc/1999-4923; https://doaj.org/article/75e44584cd034f3682bd53172ae24745 |
| DOI: | 10.3390/pharmaceutics16040538 |
| الاتاحة: | https://doi.org/10.3390/pharmaceutics16040538 https://doaj.org/article/75e44584cd034f3682bd53172ae24745 |
| رقم الانضمام: | edsbas.6166F6B9 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19994923 |
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| DOI: | 10.3390/pharmaceutics16040538 |