Academic Journal
Lack of serotonin 5-HT2B receptor alters proliferation and network volume of interstitial cells of Cajal in vivo.
| العنوان: | Lack of serotonin 5-HT2B receptor alters proliferation and network volume of interstitial cells of Cajal in vivo. |
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| المؤلفون: | Tharayil, V. S., Wouters, M. M., Stanich, J. E., Roeder, J. L., Lei, S., Beyder, A., Gomez-Pinilla, P. J., Gershon, M. D., Maroteaux, Luc, Gibbons, S. J., Farrugia, Gianrico |
| المساهمون: | Enteric Neuroscience Program, Miles and Shirley Fiterman, Mayo Clinic, Department of Pathology and Cell Biology, Columbia University New York, Institut du Fer à Moulin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Physiology and Biomedical Engineering, This work was supported and funded by NIH DK57061 (GF) and NS12969 (MDG) and from the Centre National de la Recherche Scientifique, the Institut National de la Sante' et de la Recherche Me'dicale, the Universite' Pierre et Marie Curie, and by grants from the Fondation de France, the Fondation pour la Recherche Me'dicale, the Association pour la Recherche contre le Cancer, the French ministry of research (Agence Nationale pour la Recherche), the Federation des Maladies Orphelines, and the European Union (LM). |
| المصدر: | ISSN: 1350-1925. |
| بيانات النشر: | HAL CCSD Wiley |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | serotonin receptors, cellular plasticity, gastrointestinal motility, kit, mouse jejunum, MESH: Cell Proliferation, MESH: Microscopy, Confocal, MESH: Gastrointestinal Transit, MESH: Immunohistochemistry, MESH: Interstitial Cells of Cajal, MESH: Jejunum, MESH: Mice, Knockout, MESH: Microdissection, MESH: Myenteric Plexus, MESH: Nerve Net, MESH: Neuronal Plasticity, MESH: Proto-Oncogene Proteins c-kit, MESH: Receptor, Serotonin, 5-HT2B, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: Animals, MESH: Cells, Cultured, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | International audience ; BACKGROUND: Normal gastrointestinal motility requires intact networks of interstitial cells of Cajal (ICC). Interstitial cells of Cajal numbers are maintained by a balance between cell loss factors and survival/trophic/growth factors. Activation of 5-HT(2B) receptors expressed on ICC increases ICC proliferation in vitro. It is not known whether 5-HT(2B) receptors on ICC are activated in vivo. The aims of this study were to investigate if adult ICC proliferate, whether the proliferation of ICC in vivo is affected by knocking out the 5-HT(2B) receptor, and if alterations in proliferation affect ICC networks. METHODS: Proliferating ICC were identified by immunoreactivity for Ki67 in both the myenteric and deep muscular plexus regions of the jejunum in mice with a targeted insertion of a neomycin resistance cassette into the second coding exon of the htr2b receptor gene. KEY RESULTS: Adult ICC do proliferate. The number of proliferating ICC was lower in the myenteric plexus region of Htr2b(-/-) compared to Htr2b(+/+) mice. The volume of Kit-positive ICC was 30% lower in the myenteric plexus region and 40% lower in the deep muscular plexus region in Htr2b(-/-) mice where the number of ICC was also reduced. CONCLUSIONS & INFERENCES: Interstitial cells of Cajal proliferate in adult mice and activation of 5-HT(2B) receptors results in increased proliferation of ICC in vivo. Furthermore, lack of 5-HT(2B) receptor signaling reduces the density of ICC networks in mature mice. These data suggest that 5-HT(2B) receptor signaling is required for maintenance of ICC networks, adding 5-HT to the growing number of factors shown to regulate ICC networks. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/19941613; inserm-00996753; https://www.hal.inserm.fr/inserm-00996753; PUBMED: 19941613; PUBMEDCENTRAL: PMC2852486 |
| DOI: | 10.1111/j.1365-2982.2009.01435.x |
| الاتاحة: | https://www.hal.inserm.fr/inserm-00996753 https://doi.org/10.1111/j.1365-2982.2009.01435.x |
| رقم الانضمام: | edsbas.614ACABA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-2982.2009.01435.x |
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