Academic Journal
Enhanced Patency and Endothelialization of Small-Caliber Vascular Grafts Fabricated by Coimmobilization of Heparin and Cell-Adhesive Peptides
| العنوان: | Enhanced Patency and Endothelialization of Small-Caliber Vascular Grafts Fabricated by Coimmobilization of Heparin and Cell-Adhesive Peptides |
|---|---|
| المساهمون: | College of Medicine, Dept. of Thoracic & Cardiovascular Surgery, Won Sup Choi, Yoon Ki Joung, Yunki Lee, Jin Woo Bae, Han Ki Park, Young Hwan Park, Jong-Chul Park, Ki Dong Park, Park, Young Hwan, Park, Han Ki, Park, Jong Chul |
| بيانات النشر: | American Chemical Society United States |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Animals, Aorta, Abdominal/drug effects, Abdominal/growth & development, Blood Vessel Prosthesis, Cell Adhesion/drug effects, Cell Proliferation/drug effects, Heparin/chemistry, Heparin/therapeutic use, Human Umbilical Vein Endothelial Cells, Humans, Oligopeptides/chemistry, Oligopeptides/therapeutic use, Polyurethanes/chemistry, Polyurethanes/therapeutic use, Rabbits, Thrombosis/pathology, Thrombosis/therapy, antithrombogenic activities, cell-adhesive peptide, endothelialization, heparin, polyurethane, vascular graft |
| الوصف: | The clinical utility of a small-caliber vascular graft is still limited, owing to the occlusion of graft by thrombosis and restenosis. A small-caliber vascular graft (diameter, 2.5 mm) fabricated by electrospinning with a polyurethane (PU) elastomer (Pellethane) and biofunctionalized with heparin and two cell-adhesive peptides, GRGDS and YIGSR, was developed for the purpose of preventing the thrombosis and restenosis through antithrombogenic activities and endothelialization. The vascular grafts showed slightly reduced adhesion of platelets and significantly decreased adsorption of fibrinogen. In vitro studies demonstrated that peptide treatment on a vascular graft enhanced the attachment of human umbilical vein endothelial cells (HUVECs), and the presence of heparin and peptides on the graft significantly increased the proliferation of HUVECs. In vivo implantation of heparin/peptides coimmobilized graft (PU-PEG-Hep/G+Y) and PU (control) grafts was performed using an abdominal aorta rabbit model for 60 days followed by angiographic monitoring and explanting for histological analyses. The patency was significantly higher for the modified PU grafts (71.4%) compared to the PU grafts (46.2%) at 9 weeks after implantation. The nontreated PU grafts showed higher levels of 慣-SMA expression compared to the modified grafts, and for both samples, the proximal and distal regions expressed higher levels compared to the middle region of the grafts. Moreover, immobilization of heparin and peptides and adequate porous structure were found to play important roles in endothelialization and cellular infiltration. Our results strongly encourage that the development of small-caliber vascular grafts is feasible. ; restriction |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 4336~4346 |
| اللغة: | unknown |
| تدمد: | 1944-8244 1944-8252 26824876 |
| Relation: | ACS APPLIED MATERIALS & INTERFACES; J00004; OAK-2016-01472; https://ir.ymlib.yonsei.ac.kr/handle/22282913/146597; T201600942; ACS APPLIED MATERIALS & INTERFACES, Vol.8(7) : 4336-4346, 2016 |
| DOI: | 10.1021/acsami.5b12052 |
| الاتاحة: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/146597 https://doi.org/10.1021/acsami.5b12052 http://pubs.acs.org/doi/abs/10.1021/acsami.5b12052 |
| Rights: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ |
| رقم الانضمام: | edsbas.61133835 |
| قاعدة البيانات: | BASE |
| تدمد: | 19448244 19448252 26824876 |
|---|---|
| DOI: | 10.1021/acsami.5b12052 |